Anti-KV3.1b (KCNC1) Antibody | Alomone Labs APC-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV3.1b (KCNC1) Antibody

catalog :

APC-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Dzyubenko E, Fleischer M, Manrique Castano D, Borbor M, Kleinschnitz C, Faissner A, et al. Inhibitory control in neuronal networks relies on the extracellular matrix integrity. Cell Mol Life Sci. 2021;78:5647-5663 pubmed publisher
Reijntjes D, Lee J, Park S, Schubert N, van Tuinen M, Vijayakumar S, et al. Sodium-activated potassium channels shape peripheral auditory function and activity of the primary auditory neurons in mice. Sci Rep. 2019;9:2573 pubmed publisher
Munch A, Saljic A, Boddum K, Grunnet M, Hougaard C, Jespersen T. Pharmacological rescue of mutated Kv3.1 ion-channel linked to progressive myoclonus epilepsies. Eur J Pharmacol. 2018;833:255-262 pubmed publisher
Jukkola P, Gu Y, Lovett Racke A, Gu C. Suppression of Inflammatory Demyelinaton and Axon Degeneration through Inhibiting Kv3 Channels. Front Mol Neurosci. 2017;10:344 pubmed publisher
Éltes T, Kirizs T, Nusser Z, Holderith N. Target Cell Type-Dependent Differences in Ca2+ Channel Function Underlie Distinct Release Probabilities at Hippocampal Glutamatergic Terminals. J Neurosci. 2017;37:1910-1924 pubmed publisher
Wolff M, Czorlich P, Nagaraj C, SchnÃ¶bel Ehehalt R, Li Y, Kwapiszewska G, et al. Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study. Neurosci Res. 2016;109:16-27 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KV3.1b (KCNC1) Antibody (#APC-014), (1:200).2. Anti-KV3.1b (KCNC1) Antibody, preincubated with Kv3.1b/KCNC1 Blocking Peptide (#BLP-PC014).

image 2 :

Peptide CKESPVIAKYMPTEAVRVT corresponding to amino acid residues 567-585 of rat KV3.1b (AccessionP25122). Intracellular C-terminus.

image 3 :

Co-localization of KV3.1b and KIF5 in rat hippocampal neurons - Immunocytochemical staining of rat hippocampal neurons with Anti-KV3.1b (KCNC1) Antibody (APC-014) and anti-KIF5 antibody. Arrows in right panel depict colocalization between the two proteins.Adapted from Barry J. et al. (2013) with permission of The Company of Biologists LTD.

product information

CAT :

APC-014

SKU :

APC-014-CF_0.2 ml

Product Name :

Anti-KV3.1b (KCNC1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P25122

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC014

Homology :

Mouse, human, dog - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CKESPVIAKYMPTEAVRVT, corresponding to amino acid residues 567-585 of rat KV3.1b

Product Page - Scientific background :

KV3.1 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins KV3.2, KV3.3 and KV3.4 they constitute the Shaw type subfamily family.1As with all KV channels, KV3.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional Kv channel is a tetramer that can either be a homomer or a heteromer of KV3 subunits.KV3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.3KV3.1 is highly expressed in the brain but has been also detected in peripheral organs such as lung skeletal muscle and testis.KV3.1 and KV3.2 are highly enriched in neurons that fire at high frequencies, such as fast spiking interneurons of the cortex and hippocampus and neurons in the globus pallidus. Their unusually rapid activation and deactivation rates allow channels containing KV3.1 and KV3.2 subunits to repolarize action potentials quickly thus minimizing the rate of recovery of sodium channel inactivation.3Lack of KV3.1 channel subunits is mainly responsible for constitutively increased locomotor activity and sleep loss.2

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily C member 1, NGK2, RAW2

Short Description :

A Rabbit Polyclonal Antibody to KV3.1b (KCNC1) Channel

Long Description :

Anti-KV3.1b (KCNC1) Antibody (#APC-014) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize KV3.1b from rat, mouse, and human samples.

Negative Control :

BLP-PC014

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily C member 1, NGK2, RAW2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNC1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage