Anti-KV2.1 Antibody | Alomone Labs APC-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV2.1 Antibody

catalog :

APC-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 8

Reference
Joseph L, Reyes M, Homan E, Gowen B, Avula U, Goulbourne C, et al. The mitochondrial calcium uniporter promotes arrhythmias caused by high-fat diet. Sci Rep. 2021;11:17808 pubmed publisher
Lee M, Nahorski M, Hockley J, Lu V, Ison G, Pattison L, et al. Human Labor Pain Is Influenced by the Voltage-Gated Potassium Channel KV6.4 Subunit. Cell Rep. 2020;32:107941 pubmed publisher
Greitzer Antes D, Xie L, Qin T, Xie H, Zhu D, Dolai S, et al. Kv2.1 clusters on β-cell plasma membrane act as reservoirs that replenish pools of newcomer insulin granule through their interaction with syntaxin-3. J Biol Chem. 2018;293:6893-6904 pubmed publisher
Fu L, Lv Y, Zhong Y, He Q, Liu X, Du L. Tyrosine phosphorylation of Kv1.5 is upregulated in intrauterine growth retardation rats with exaggerated pulmonary hypertension. Braz J Med Biol Res. 2017;50:e6237 pubmed publisher
Justice J, Schulien A, He K, Hartnett K, Aizenman E, Shah N. Disruption of KV2.1 somato-dendritic clusters prevents the apoptogenic increase of potassium currents. Neuroscience. 2017;354:158-167 pubmed publisher
Regnier G, Bocksteins E, Van de Vijver G, Snyders D, Van Bogaert P. The contribution of Kv2.2-mediated currents decreases during the postnatal development of mouse dorsal root ganglion neurons. Physiol Rep. 2016;4: pubmed publisher
Wolff M, Czorlich P, Nagaraj C, SchnÃ¶bel Ehehalt R, Li Y, Kwapiszewska G, et al. Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study. Neurosci Res. 2016;109:16-27 pubmed publisher
Peltola M, Kuja Panula J, Liuhanen J, VÃµikar V, Piepponen P, Hiekkalinna T, et al. AMIGO-Kv2.1 Potassium Channel Complex Is Associated With Schizophrenia-Related Phenotypes. Schizophr Bull. 2016;42:191-201 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KV2.1 Antibody (#APC-012), (1:500).2. Anti-KV2.1 Antibody, preincubated with KV2.1 Blocking Peptide (BLP-PC012).

image 2 :

Expression of KV2.1 channel in rat pancreas - Immunohistochemical staining of rat paraffin embedded pancreas sections using Anti-KV2.1 Antibody (#APC-012), (1:50), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody. Endocrine (Islets of Langerhans) and exocrine areas of pancreas section are shown. Strong and highly specific KV2.1 staining is evident only in endocrine cells (red). Hoechst 33342 is used as the counterstain (blue).

image 3 :

Western blot analysisof rat brain membranes: - 1.Anti-KV2.1Antibody (#APC-012) (1:500).2. Anti-KV2.1 Antibody preincubated with the control peptide antigen.

product information

CAT :

APC-012

SKU :

APC-012-CF_0.2 ml

Product Name :

Anti-KV2.1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P15387

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC012

Homology :

Mouse - identical; human, rabbit - 15/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(CY)HMLPGGGAHGSTRDQSI, corresponding to amino acid residues 841-857 of rat KV2.1

Product Page - Scientific background :

KV2.1 is a member of the voltage-gated K+ channel superfamily. Together with the closely related KV2.2 protein they form the KV2 subfamily also known as Shab.1As with all KV channels, KV2.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N and C termini. The functional KV channel is a tetramer that can either be a homotetramer or a heteromer of KV2.1 and KV2.2 subunits.Both KV2.1 and KV2.2 channels are known as delayed rectifiers that is, channels that are activated by changes in membrane potential (depolarization) but inactivate very slowly. The current they form is known as IK or IDR. Accessory subunits such as KChaP and the electrically silent a subunits KV8 and KV9 can modulate biochemical and biophysical properties of KV2.1.2KV2.1 is widely expressed throughout the body including brain, lung, pancreas, skeletal muscle and pulmonary artery.2 The main function of KV2.1 is to maintain membrane potential and to modulate the electrical excitability in neurons and muscle. In rat pulmonary artery it probably mediates hypoxic pulmonary vasoconstriction together with the KV9.3 subunit.3 Several toxins from spider venoms are potent blockers (affecting the channels in the nanomolar range) of KV2.1 channels. Among these the most potent and selective are Stromatoxin-1 (#STS-350), (12.7 nM)4 and Hanatoxin (42 nM).4

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

KCNB1, DRK1, Delayed rectifier potassium channel 1, Potassium voltage-gated channel subfamily B member 1

Short Description :

A Rabbit Polyclonal Antibody to KV2.1 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat KV2.1 channel. Anti-KV2.1 Antibody (#APC-012) can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize KV2.1 from rat, human, and mouse samples.

Negative Control :

BLP-PC012

Positive Control :

Synonyms :

KCNB1, DRK1, Delayed rectifier potassium channel 1, Potassium voltage-gated channel subfamily B member 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNB1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

IgG fraction purified on immobilized Protein A.

UNSPSC :

41116161

Cited Application :

IP ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage