Guinea pig Anti-KV2.1 Antibody | Alomone Labs APC-012-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-KV2.1 Antibody

catalog :

APC-012-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Fan F, Tian H, Geng J, Deng J, Liu Y, Chen C, et al. Mechanism of Beraprost Effects on Pulmonary Hypertension: Contribution of Cross-Binding to PGE2 Receptor 4 and Modulation of O2 Sensitive Voltage-Gated K+ Channels. Front Pharmacol. 2018;9:1518 pubmed publisher
Greitzer Antes D, Xie L, Qin T, Xie H, Zhu D, Dolai S, et al. Kv2.1 clusters on β-cell plasma membrane act as reservoirs that replenish pools of newcomer insulin granule through their interaction with syntaxin-3. J Biol Chem. 2018;293:6893-6904 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Guinea pig Anti-KV2.1 Antibody (#APC-012-GP), (1:500).3,4. Guinea pig Anti-KV2.1 Antibody, preincubated with Kv2.1 Blocking Peptide (#BLP-PC012).

image 2 :

Expression of KV2.1 channel in rat hippocampus - Immunohistochemical staining of rat hippocampal CA1 region using Guinea pig Anti-KV2.1 Antibody (#APC-012-GP), (1:400). A. KV2.1 staining (green) appears in the pyramidal layer (P) and in stratum oriens (SO) interneurons (horizontal arrows). B. Nuclei staining using DAPI as the counterstain (blue). C. Merged image of panels A and B.

product information

CAT :

APC-012-GP

SKU :

APC-012-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-KV2.1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P15387

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PC012

Homology :

Mouse - identical; human - 15/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(CY)HMLPGGGAHGSTRDQSI, corresponding to amino acid residues 841-857 of rat KV2.1

Product Page - Scientific background :

Voltage gated K+ channels are involved in diverse physiological processes ranging from repolarization of neuronal and cardiac action potential, regulation of Ca2+ signaling and cell volume to driving cellular proliferation and migration.KV2.1 consists of four α-subunits, each containing six transmembrane α-helical segments, S1-S6, and a membrane-re-entering P-loop, which are arranged circumferentially around a central pore. This ion conduction pore is lined by four S5-P-S6 sequences. The four first segments(1-4), each containing four positively charged arginine residues in the Segment number 4 helix, act as voltage sensor domains and "gate" the pore by "pulling" on the segments 4 and 5 linker.KV2.1, encoded by the KCNB1 gene, is a classical delayed rectifier channel that is involved in neuronal repolarization. Its function can be diversified through heteromultimerization with "silent" KV units, which modify inactivation, trafficking, drug sensitivity and expression.Inhibition of KV2.1 in pancreatic β-cells enhances insulin secretion, suggesting a potential therapeutic strategy for type 2 diabetes. This effect is presumed to occur through non-conducting functions - namely, a physical interaction with syntaxin (a component of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor complex) that facilitates vesicle fusion1.Recently, a "de novo" pathogenic KV2.1 variant was discovered in a family suffering from idiopathic epileptic encephalopathy. This variant is a missense mutation in the coding region of the KCNB1 gene. Expression of the V378A variant results in voltage-activated currents that are sensitive to the selective KV2 channel blocker guangxitoxin-1E. Currents through the mutant channel are non-selective among monovalent cations2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

KCNB1, DRK1, Delayed rectifier potassium channel 1, Potassium voltage-gated channel subfamily B member 1

Short Description :

A Guinea Pig Polyclonal Antibody to KV2.1 Channel

Long Description :

Guinea pig Anti-KV2.1 Antibody (#APC-012-GP, formerly AGP-109) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV2.1 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-KV2.1 Antibody (#APC-012) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PC012

Positive Control :

Synonyms :

KCNB1, DRK1, Delayed rectifier potassium channel 1, Potassium voltage-gated channel subfamily B member 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNB1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage