Anti-KV3.2 (KCNC2) Antibody | Alomone Labs APC-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV3.2 (KCNC2) Antibody

catalog :

APC-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Nabel A, Callan A, Gleiss S, Kladisios N, Leibold C, Felmy F. Distinct Distribution Patterns of Potassium Channel Sub-Units in Somato-Dendritic Compartments of Neurons of the Medial Superior Olive. Front Cell Neurosci. 2019;13:38 pubmed publisher
Wolff M, Czorlich P, Nagaraj C, SchnÃ¶bel Ehehalt R, Li Y, Kwapiszewska G, et al. Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study. Neurosci Res. 2016;109:16-27 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KV3.2 (KCNC2) Antibody (#APC-011), (1:200).2. Anti-KV3.2 (KCNC2) Antibody, preincubated with Kv3.2/KCNC2 Blocking Peptide (#BLP-PC011).

image 2 :

Western blot analysisof rat brain membranes: - 1.Anti-KV3.2(KCNC2)Antibody (#APC-011) (1:200).2. Anti-KV3.2 (KCNC2) Antibody preincubated with the negative control antigen.

image 3 :

Western blot analysis of rat brain membranes (lanes 1 and 4) mouse brain membranes (lanes 2 and 5) and rat spleen membranes (lanes 3 and 6): - 1-3. Anti-GPR84 (extracellular) Antibody (#AGR-052) (1:200).4-6. Anti-GPR84 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-011

SKU :

APC-011-CF_0.2 ml

Product Name :

Anti-KV3.2 (KCNC2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P22462

Applications :

IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC011

Homology :

Human, mouse - 19 out of 21 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

DLGGKRLGIEDAAGLGGPDGK(C), corresponding to amino acid residues 184-204 of rat KV3.2

Product Page - Scientific background :

KV3.2 is a member of the voltage-gated K+ channel superfamily. Together with the related proteins KV3.1, KV3.3 and KV3.4 they constitute the Shaw type subfamily family.1As with all KV channels, KV3.2 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C-termini. The functional KV channel is a tetramer that can either be a homomer or a heteromer of KV3 subunits.KV3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing.2,3KV3.2 is highly expressed in the brain but has been also detected in peripheral organs such as pancreas and mesenteric artery.KV3.2 and KV3.1 are highly enriched in neurons that fire at high frequencies, such as fast-spiking interneurons of the cortex and hippocampus and neurons in the globus pallidus. Their unusually rapid activation and deactivation rates allow channels containing KV3.2 and KV3.1 subunits to repolarize action potentials quickly thus minimizing the rate of recovery of sodium channel inactivation.2,3

Applications may also work in :

IF IHC IP WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily C member 2, Shaw-like potassium channel, KSHIIIA

Short Description :

A Rabbit Polyclonal Antibody to KV3.2 (KCNC2) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV3.2. Anti-KV3.2 (KCNC2) Antibody (#APC-011) can be used in western blot, immunoprecipitation, and immunohistochemistry applications. It has been designed to recognize KV3.2 from human, rat, and mouse samples.

Negative Control :

BLP-PC011

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily C member 2, Shaw-like potassium channel, KSHIIIA

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNC2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage