Anti-GIRK2 (Kir3.2) Antibody | Alomone Labs APC-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-GIRK2 (Kir3.2) Antibody

catalog :

APC-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 29

Published Application/Species/Sample/Dilution	Reference
immunocytochemistry; human; 1:500; loading ...; fig s11a	Yoo J, Lee D, Park S, Shin H, Lee K, Kim D, et al. Trophoblast glycoprotein is a marker for efficient sorting of ventral mesencephalic dopaminergic precursors derived from human pluripotent stem cells. NPJ Parkinsons Dis. 2021;7:61 pubmed publisher
immunocytochemistry; human; 1:500; loading ...; fig 1h	Nilsson F, Storm P, Sozzi E, Hidalgo Gil D, Birtele M, Sharma Y, et al. Single-Cell Profiling of Coding and Noncoding Genes in Human Dopamine Neuron Differentiation. Cells. 2021;10: pubmed publisher
immunocytochemistry; human; 1:100; fig 1b	Paik E, O Neil A, Ng S, Sun C, Rubin L. Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture. Sci Rep. 2018;8:804 pubmed publisher
immunohistochemistry; rat; 1:1000; fig s1	Zhang L, Hernandez V, Vázquez Juárez E, Chay F, Barrio R. Thirst Is Associated with Suppression of Habenula Output and Active Stress Coping: Is there a Role for a Non-canonical Vasopressin-Glutamate Pathway?. Front Neural Circuits. 2016;10:13 pubmed publisher
	Aldrin Kirk P, Akerblom M, Cardoso T, Nolbrant S, Adler A, Liu X, et al. A novel two-factor monosynaptic TRIO tracing method for assessment of circuit integration of hESC-derived dopamine transplants. Stem Cell Reports. 2021;: pubmed publisher
	Fukusumi H, Togo K, Sumida M, Nakamori M, Obika S, Baba K, et al. Alpha-synuclein dynamics in induced pluripotent stem cell-derived dopaminergic neurons from a Parkinson's disease patient (PARK4) with SNCA triplication. FEBS Open Bio. 2021;11:354-366 pubmed publisher
	Mett A, Karbat I, Tsoory M, Fine S, Iwanir S, Reuveny E. Reduced activity of GIRK1-containing heterotetramers is sufficient to affect neuronal functions, including synaptic plasticity and spatial learning and memory. J Physiol. 2021;599:521-545 pubmed publisher
	Ke M, Chong C, Zeng H, Huang M, Huang Z, Zhang K, et al. Azoramide protects iPSC-derived dopaminergic neurons with PLA2G6 D331Y mutation through restoring ER function and CREB signaling. Cell Death Dis. 2020;11:130 pubmed publisher
	Gantner C, De Luzy I, Kauhausen J, Moriarty N, Niclis J, Bye C, et al. Viral Delivery of GDNF Promotes Functional Integration of Human Stem Cell Grafts in Parkinson's Disease. Cell Stem Cell. 2020;26:511-526.e5 pubmed publisher
	De Luzy I, Niclis J, Gantner C, Kauhausen J, Hunt C, Ermine C, et al. Isolation of LMX1a Ventral Midbrain Progenitors Improves the Safety and Predictability of Human Pluripotent Stem Cell-Derived Neural Transplants in Parkinsonian Disease. J Neurosci. 2019;39:9521-9531 pubmed publisher
	Arnold E, McMurray C, Gray R, Johnston D. Epilepsy-Induced Reduction in HCN Channel Expression Contributes to an Increased Excitability in Dorsal, But Not Ventral, Hippocampal CA1 Neurons. Eneuro. 2019;6: pubmed publisher
	Ostrovskaya O, Orlandi C, Fajardo Serrano A, Young S, Lujan R, Martemyanov K. Inhibitory Signaling to Ion Channels in Hippocampal Neurons Is Differentially Regulated by Alternative Macromolecular Complexes of RGS7. J Neurosci. 2018;38:10002-10015 pubmed publisher
	Tang X, Jiao L, Zheng M, Yan Y, Nie Q, Wu T, et al. Tau Deficiency Down-Regulated Transcription Factor Orthodenticle Homeobox 2 Expression in the Dopaminergic Neurons in Ventral Tegmental Area and Caused No Obvious Motor Deficits in Mice. Neuroscience. 2018;373:52-59 pubmed publisher
	Jovanovic V, Salti A, Tilleman H, Zega K, Jukic M, Zou H, et al. BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells. J Neurosci. 2018;38:1662-1676 pubmed publisher
	Honda I, Araki K, Honda S, Soeda F, Shin M, Misumi S, et al. Deletion of GIRK2 subunit containing GIRK channels of neurons expressing dopamine transporter decrease immobility time on forced swimming in mice. Neurosci Lett. 2018;665:140-146 pubmed publisher
	Niclis J, Gantner C, Hunt C, Kauhausen J, Durnall J, Haynes J, et al. A PITX3-EGFP Reporter Line Reveals Connectivity of Dopamine and Non-dopamine Neuronal Subtypes in Grafts Generated from Human Embryonic Stem Cells. Stem Cell Reports. 2017;9:868-882 pubmed publisher
	Kikuchi T, Morizane A, Doi D, Magotani H, Onoe H, Hayashi T, et al. Human iPS cell-derived dopaminergic neurons function in a primate Parkinson's disease model. Nature. 2017;548:592-596 pubmed publisher
	May L, Anggono V, Gooch H, Jang S, Matusica D, Kerbler G, et al. G-Protein-Coupled Inwardly Rectifying Potassium (GIRK) Channel Activation by the p75 Neurotrophin Receptor Is Required for Amyloid β Toxicity. Front Neurosci. 2017;11:455 pubmed publisher
	Roncacé V, Burattini C, Stagni F, Guidi S, Giacomini A, Emili M, et al. Neuroanatomical alterations and synaptic plasticity impairment in the perirhinal cortex of the Ts65Dn mouse model of Down syndrome. Neurobiol Dis. 2017;106:89-100 pubmed publisher
	Marron Fernandez de Velasco E, Zhang L, N Vo B, Tipps M, Farris S, Xia Z, et al. GIRK2 splice variants and neuronal G protein-gated K+ channels: implications for channel function and behavior. Sci Rep. 2017;7:1639 pubmed publisher
	Rivetti di Val Cervo P, Romanov R, Spigolon G, Masini D, Martín Montañez E, Toledo E, et al. Induction of functional dopamine neurons from human astrocytes in vitro and mouse astrocytes in a Parkinson's disease model. Nat Biotechnol. 2017;35:444-452 pubmed publisher
	Kleschevnikov A, Yu J, Kim J, Lysenko L, Zeng Z, Yu Y, et al. Evidence that increased Kcnj6 gene dose is necessary for deficits in behavior and dentate gyrus synaptic plasticity in the Ts65Dn mouse model of Down syndrome. Neurobiol Dis. 2017;103:1-10 pubmed publisher
	Stott S, Hayat S, Carnwath T, Garas S, Sleeman J, Barker R. CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson's disease. PLoS ONE. 2017;12:e0171748 pubmed publisher
	Fjodorova M, Torres E, Dunnett S. Transplantation site influences the phenotypic differentiation of dopamine neurons in ventral mesencephalic grafts in Parkinsonian rats. Exp Neurol. 2017;291:8-19 pubmed publisher
	Joshi K, Shen L, Michaeli A, Salter M, Thibault Messier G, Hashmi S, et al. Infantile spasms in down syndrome: Rescue by knockdown of the GIRK2 channel. Ann Neurol. 2016;80:511-21 pubmed publisher
	Booker S, Althof D, Gross A, Loreth D, MÃ¼ller J, Unger A, et al. KCTD12 Auxiliary Proteins Modulate Kinetics of GABAB Receptor-Mediated Inhibition in Cholecystokinin-Containing Interneurons. Cereb Cortex. 2017;27:2318-2334 pubmed publisher
	Brandalise F, Lujan R, Leone R, Lodola F, Cesaroni V, Romano C, et al. Distinct expression patterns of inwardly rectifying potassium currents in developing cerebellar granule cells of the hemispheres and the vermis. Eur J Neurosci. 2016;43:1460-73 pubmed publisher
	Lelos M, Morgan R, Kelly C, Torres E, Rosser A, Dunnett S. Amelioration of non-motor dysfunctions after transplantation of human dopamine neurons in a model of Parkinson's disease. Exp Neurol. 2016;278:54-61 pubmed publisher
	Robertson D, Sleno R, Nagi K, PÃ©trin D, HÃ©bert T, Pineyro G. Design and construction of conformational biosensors to monitor ion channel activation: A prototype FlAsH/BRET-approach to Kir3 channels. Methods. 2016;92:19-35 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-GIRK2 (Kir3.2) Antibody (#APC-006), (1:200).2. Anti-GIRK2 (Kir3.2) Antibody, preincubated with GIRK2 (Kir3.2) Blocking peptide (#BLP-PC006).

image 2 :

Expression of Kir3.2 in mouse brain - Immunohistochemical staining of perfusion-fixed frozen mouse substantia nigra pars compacta sections using Anti-GIRK2 (Kir3.2) Antibody (#APC-006), (1:400). GIRK2 staining (red) appears in cells and processes along the pars compacta of the mouse substantia nigra (horizontal arrows) and in the pars reticulata (vertical arrows). Cell nuclei are stained with DAPI (blue).

image 3 :

GST fusion protein with the sequence ELANRAEVPLSWSVS SKLNQHAELETEEEEKNPEELTERNG corresponding to residues 374-414 of mouse Kir3.2 (Accession P48542). Intracellular C-terminal part.

product information

CAT :

APC-006

SKU :

APC-006-CF_0.2 ml

Product Name :

Anti-GIRK2 (Kir3.2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P48542

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC006

Homology :

Rat - 40/41 amino acid residues identical; golden hamster - 39/41 amino acid residues identical; human - 37/41 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by DNA sequence and SDS-PAGE

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

GST fusion protein with the sequence ELANRAEVPLSWSVS SKLNQHAELETEEEEKNPEELTERNG, corresponding to residues 374-414 of mouse Kir3.2

Product Page - Scientific background :

Kir3.2 (or G-protein regulated inward-rectifier K+ channel, GIRK2) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heterotetramers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir3.2 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Gα-GTP from the Gbg dimer. The latter directly binds to Kir3 and activates the channel.2,3Kir3.2 is mainly expressed in the brain, where it co-assembles with Kir3.1 (GIRK1) or Kir3.3 (GIRK3) and mediates the inhibitory effects of many neurotransmitters including opioid, adrenergic, muscarinic, dopaminergic and GABAergic neurotransmitters.2,3Point mutations in the mouse Kir3.2 channel cause the weaver (wv) phenotype, a neurological abnormality characterized by the abnormal 'weaving' of the mice when they walk, hence the name weaver which is due to a substantial loss of cerebellar granule neurons. These mice also display mild local motor hyperactivity, presumably caused by the degeneration of dopaminergic neurons in the substantia nigra, spontaneous seizures and male sterility.1A peptide toxin originating from the Apis mellifera bee venom, Tertiapin (#STT-250) was shown to be a potent blocker of Kir3.2 containing channels (7 nM for Kir3.2 alone and 5.4 nM for the Kir3.1/3.2 combination).4

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

G protein-activated inward rectifier potassium channel 2, Kcnj6

Short Description :

A Rabbit Polyclonal Antibody to GIRK2 (Kir3.2) Channel

Long Description :

Anti-GIRK2 (Kir3.2) Antibody (#APC-006) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize Kir3.2 from rat, mouse, and human samples.

Negative Control :

BLP-PC006

Positive Control :

Synonyms :

G protein-activated inward rectifier potassium channel 2, Kcnj6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC LCI

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

3 µg fusion protein per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage