Guinea pig Anti-GIRK2 (Kir3.2) Antibody | Alomone Labs APC-006-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-GIRK2 (Kir3.2) Antibody

catalog :

APC-006-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Banerjee A, Lu Y, Do K, Mize T, Wu X, Chen X, et al. Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases. Front Cell Neurosci. 2021;15:629279 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4): - 1,2. Guinea pig Anti-GIRK2 (Kir3.2) Antibody (#APC-006-GP), (1:400).3,4. Guinea pig Anti-GIRK2 (Kir3.2) Antibody, preincubated with GIRK2/Kir3.2 Blocking Peptide (#BLP-PC006). Following a screen of several secondary antibodies, the following were used for western blot analysis:Anti-Guinea pig IgG (Sigma #A7289 or Jackson ImmunoResearch #106-035-006).

image 2 :

Multiplex staining of GIRK2 (Kir3.2) and LRRK2 in mouse substantia nigra pars compacta - Immunohistochemical staining of immersion-fixed, free floating mouse brain frozen sections using Guinea pig Anti-GIRK2 (Kir3.2) Antibody (#APC-006-GP), (1:200) and rabbit Anti-LRRK2 Antibody (#ANR-102), (1:200). A. Kir3.2 staining (red) appears in profiles of dopaminergic neurons (arrows). B. LRRK2 staining (green) is detected in several cell bodies in the SNC. C. Merge of the two images demonstrates colocalization in several neurons (arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APC-006-GP

SKU :

APC-006-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-GIRK2 (Kir3.2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P48542

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PC006

Homology :

Rat - 40/41, human - 37/41 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by DNA sequence and SDS-PAGE

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

GST fusion protein with the sequence ELANRAEVPLSWSVS SKLNQHAELETEEEEKNPEELTERNG, corresponding to residues 374-414 of mouse Kir3.2

Product Page - Scientific background :

Inward rectifying K+ (Kir) channels generate under physiological conditions, large K+ conductance at potentials negative to Ek but permit less current flow at potentials positive to Ek.Kir3.2 (also known as GIRK2 or KCNJ6) belongs to a G-protein gated subfamily of Kir channels. Kir3.2 is one of four subunits (Kir3.1, Kir3.3 and Kir3.4) that form functional tetrameric KG channel assemblies. KG channels are activated by GTP (GTPi) in the presence of an agonist or by intracellular GTPγS in the absence of an agonist. There are at least four different isoforms of Kir3.2 which are generated by alternative splicing from a single gene. Kir3.2 isoforms exhibit differential expression patterns in various tissues such as brain, pituitary, pancreas, and testis, where they usually coincide with the expression of other Kir3.x subunits.Kir3.2 is expressed on the cell surface due to two forward trafficking signal in its cytoplasmic region. One is an ER export signal in its NH2 terminus and the other is a post-ER trafficking signal that is composed of a cluster of acidic residues in the COOH terminus.Knockout of Kir3.2 is accompanied by a decrease in Kir3.1 protein expression in the brain, the development of spontaneous seizures, and a range of phenotypes related to sporadic seizures1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

G protein-activated inward rectifier potassium channel 2, Kcnj6

Short Description :

A Guinea Pig Polyclonal Antibody to GIRK2 (Kir3.2) Channel

Long Description :

Guinea pig Anti-GIRK2 (Kir3.2) Antibody (#APC-006-GP, formerly AGP-013) raised in guinea pigs is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GIRK2 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-GIRK2 (Kir3.2) Antibody (#APC-006) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PC006

Positive Control :

Synonyms :

G protein-activated inward rectifier potassium channel 2, Kcnj6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

3 µg fusion protein per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200-1:400

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage