Anti-KV1.5 (KCNA5) Antibody | Alomone Labs APC-004 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV1.5 (KCNA5) Antibody

catalog :

APC-004

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 11

Reference
Stulajterova R, Medvecky L, Giretova M, Sopcak T, Luptakova L, Bures R, et al. Characterization of Tetracalcium Phosphate/Monetite Biocement Modified by Magnesium Pyrophosphate. Materials (Basel). 2022;15: pubmed publisher
Du Y, Wang T, Guo J, Li W, Yang T, Szendrey M, et al. Kv1.5 channels are regulated by PKC-mediated endocytic degradation. J Biol Chem. 2021;296:100514 pubmed publisher
Anton R, Ghenghea M, Ristoiu V, Gattlen C, Suter M, Cojocaru P, et al. Potassium Channels Kv1.3 and Kir2.1 But Not Kv1.5 Contribute to BV2 Cell Line and Primary Microglial Migration. Int J Mol Sci. 2021;22: pubmed publisher
Milton A, Wang T, Li W, Guo J, Zhang S. Mechanical stretch increases Kv1.5 current through an interaction between the S1-S2 linker and N-terminus of the channel. J Biol Chem. 2020;295:4723-4732 pubmed publisher
Kilfoil P, Chapalamadugu K, Hu X, Zhang D, Raucci F, Tur J, et al. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits. J Mol Cell Cardiol. 2019;137:93-106 pubmed publisher
Lamothe S, Hogan Cann A, Li W, Guo J, Yang T, Tschirhart J, et al. The N terminus and transmembrane segment S1 of Kv1.5 can coassemble with the rest of the channel independently of the S1-S2 linkage. J Biol Chem. 2018;293:15347-15358 pubmed publisher
Mondejar Parreño G, Callejo M, Barreira B, Morales Cano D, Esquivel Ruiz S, Moreno L, et al. miR-1 is increased in pulmonary hypertension and downregulates Kv1.5 channels in rat pulmonary arteries. J Physiol. 2019;597:1185-1197 pubmed publisher
Fu L, Lv Y, Zhong Y, He Q, Liu X, Du L. Tyrosine phosphorylation of Kv1.5 is upregulated in intrauterine growth retardation rats with exaggerated pulmonary hypertension. Braz J Med Biol Res. 2017;50:e6237 pubmed publisher
de la Cruz A, Vera Zambrano A, Peraza D, Valenzuela C, Zapata J, Perez Chacon G, et al. Fludarabine Inhibits KV1.3 Currents in Human B Lymphocytes. Front Pharmacol. 2017;8:177 pubmed publisher
Tur J, Chapalamadugu K, Katnik C, Cuevas J, Bhatnagar A, Tipparaju S. Kv?1.1 (AKR6A8) senses pyridine nucleotide changes in the mouse heart and modulates cardiac electrical activity. Am J Physiol Heart Circ Physiol. 2017;312:H571-H583 pubmed publisher
JimÃ©nez PÃ©rez L, Cidad P, Ãlvarez Miguel I, Santos HipÃ³lito A, Torres Merino R, Alonso E, et al. Molecular Determinants of Kv1.3 Potassium Channels-induced Proliferation. J Biol Chem. 2016;291:3569-80 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KV1.5 (KCNA5) Antibody (#APC-004), (1:100).2. Anti-KV1.5 (KCNA5) Antibody, preincubated with Kv1.5/KCNA5 Blocking Peptide (#BLP-PC004).

image 2 :

Western blot analysisof rat brain membranes: - 1.Anti-KV1.5(KCNA5)Antibody (#APC-004) (1:100).2. Anti-KV1.5 (KCNA5) Antibody preincubated with the control peptide antigen.

product information

CAT :

APC-004

SKU :

APC-004-CF_0.2 ml

Product Name :

Anti-KV1.5 (KCNA5) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61762

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC004

Homology :

Rat - 86/90 amino acid residues identical; rabbit - 71/90 amino acid residues identical; human - 70/90 amino acid residues identical; bovine, dog - 66/90 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by DNA sequence and SDS-PAGE

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

DSIRRGSCPLEKCHLKAKSNVDLRRSLYALCLDTS RETDL, corresponding to amino acid residues 513-602 of mouse KV1.5
HRETDHEEQAALKEEQGIQRRESGLDTGGQRKVSCSKAS
FHKTGGPLEST
GST fusion protein with the sequence

Product Page - Scientific background :

KV1.5 is a mammalian voltage dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.5 was first cloned from the rat brain.1 Eight Shaker related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.The structure of KV1.5 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2The channel is expressed in cardiac and smooth muscle tissue (colon, aorta, stomach and pulmonary artery) as well as in neurons and kidney.2 A loss-of-function mutation in the gene encoding the channel was found in atrial fibrillation patients, stressing its role as a cardiac action potential regulator.3The functional channel is considered transient (A-type) channel and shows prominent inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.KV1.5 channels are sensitive to high doses of TEA (330 mM) and low doses of 4-AP (0.27 mM), the "classical" non-selective potassium channel blockers.2

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily A member 5

Short Description :

A Rabbit Polyclonal Antibody to KV1.5 (KCNA5) Channel

Long Description :

Anti-KV1.5 (KCNA5) Antibody (#APC-004) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize KV1.5 from human, rat, and mouse samples.

Negative Control :

BLP-PC004

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily A member 5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNA5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

3 µg fusion protein per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:100

more info or order :

Alomone Labs product webpage