Anti-KV1.5 (KCNA5)-ATTO Fluor-550 Antibody | Alomone Labs APC-004-AO product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV1.5 (KCNA5)-ATTO Fluor-550 Antibody

catalog :

APC-004-AO

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Nystoriak M, Zhang D, Jagatheesan G, Bhatnagar A. Heteromeric complexes of aldo-keto reductase auxiliary KV? subunits (AKR6A) regulate sarcolemmal localization of KV1.5 in coronary arterial myocytes. Chem Biol Interact. 2017;276:210-217 pubmed publisher

image

image 1 :

Expression of KV1.5 in mouse cerebellum - Immunohistochemical staining of perfusion fixed, free-floating frozen mouse brain sections using Anti-KV1.5 (KCNA5)-ATTO Fluor-550 Antibody (#APC-004-AO), (1:50), (red). Staining was detected in cerebellar Bergmann glial cells (arrows). DAPI is used as the counterstain (blue). G = granule layer, P = Purkinje layer, M = molecular layer.

image 2 :

Expression of KV1.5 in mouse cerebellum - Immunohistochemical staining of perfusion fixed free-floating frozen mouse brain sections usingAnti-KV1.5 (KCNA5)-ATTO-550 Antibody(#APC-004-AO) (1:50) (red). Staining was detected in cerebellar Bergmann glial cells (arrows). DAPI is used as thecounterstain (blue). G = granule layer P = Purkinje layer M = molecular layer.

image 3 :

Expression of KV1.5 in rat cerebellum - Immunohistochemical staining of perfusion fixed free-floating frozen rat brain sections usingAnti-KV1.5 (KCNA5)-ATTO-550 Antibody(#APC-004-AO) (1:50) (red). Staining was detected in cerebellar Bergmann glial cells (arrows). DAPI is usedas the counterstain (blue).G = granule layer P = Purkinje layer M = molecular layer.

product information

CAT :

APC-004-AO

SKU :

APC-004-AO_50 mcl

Product Name :

Anti-KV1.5 (KCNA5)-ATTO Fluor-550 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q61762

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC004

Homology :

Rat - 86/90 amino acid residues identical; rabbit - 71/90 amino acid residues identical; human - 70/90 amino acid residues identical; bovine, dog - 66/90 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by DNA sequence and SDS-PAGE

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

DSIRRGSCPLEKCHLKAKSNVDLRRSLYALCLDTS RETDL, corresponding to amino acid residues 513-602 of mouse KV1.5
HRETDHEEQAALKEEQGIQRRESGLDTGGQRKVSCSKAS
FHKTGGPLEST
GST fusion protein with the sequence

Product Page - Scientific background :

KV1.5 is a mammalian voltage dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.5 was first cloned from the rat brain.1 Eight Shaker related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.The structure of KV1.5 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2The channel is expressed in cardiac and smooth muscle tissue (colon, aorta, stomach and pulmonary artery) as well as in neurons and kidney.2 A loss-of-function mutation in the gene encoding the channel was found in atrial fibrillation patients, stressing its role as a cardiac action potential regulator.3The functional channel is considered transient (A-type) channel and shows prominent inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.KV1.5 channels are sensitive to high doses of TEA (330 mM) and low doses of 4-AP (0.27 mM), the "classical" non-selective potassium channel blockers.2

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily A member 5

Short Description :

A Rabbit Polyclonal Antibody to KV1.5 (KCNA5) Channel Conjugated to the Fluorescent Dye ATTO-550

Long Description :

Anti-KV1.5 (KCNA5) Antibody (#APC-004) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize KV1.5 from human, rat, and mouse samples. Anti-KV1.5 (KCNA5)-ATTO Fluor-550 Antibody (#APC-004-AO) is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-KV1.5 (KCNA5)-ATTO Fluor-550 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AO

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily A member 5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNA5

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APC-004), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

3 µg fusion protein per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage