Anti-Opsin 3 (extracellular) Antibody | Alomone Labs AOR-023 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Opsin 3 (extracellular) Antibody

catalog :

AOR-023

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4):

image 2 :

Western blot analysis of rat eye lysate:

image 3 :

Western blot analysis of Malme-3 skin fibroblast cell line lysates (lanes 1 and 5), human THP-1 monocytic leukemia cell line lysate (lanes 2 and 6), human Jurkat T-cell leukemia cell line lysate (lanes 3 and 7) and human ARPE-19 retinal pigmental epithelia cell line lysate (lanes 4 and 8):

product information

CAT :

AOR-023

SKU :

AOR-023-CF_0.2 ml

Product Name :

Anti-Opsin 3 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WUK7

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-OR023

Homology :

Mouse - 14 out of 15 amino acid identical Rat, human - 12 out of 15 amino acid identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 2nd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)DIHGLG(S)TVDWRSKD, corresponding to amino acid residues 180 - 194 of mouse Opsin 3

Product Page - Scientific background :

Opsin 3 (OPN3), also known as encephalopsin or panopsin, is a member of the opsin family, which are light-sensitive G protein-coupled receptors (GPCRs) primarily known for their role in vision.1-3Opsins are found throughout the animal kingdom and while most vertebrates have a similar repertoire of 6-20 genes, mammals most commonly have eight opsins, although humans have nine.1-3Unlike Rhodopsin (or Opsin 2), the founding member of the opsin family, which is located in the retina and is essential for vision in low-light conditions, Opsin 3 has a more widespread expression pattern and functions that extend beyond traditional visual processing.1-3Opsin 3 is expressed in various tissues, including the brain, skin, testes, and lungs, among others.1-3Opsin 3 is abundantly expressed in the skin (a major light sensing organ). The function of Opsin 3 in photodetection and photosensitivity is not clear, but it appears to be based in a light-independent manner and through the interaction with MC1R, another GPCR that is crucial for the regulation of pigmentation in the skin and hair. 4An intriguing role for Opsin 3 was described in relation to regulation of energy metabolism. Opsin 3 was shown to regulate thermogenesis and lipolysis via a blue-light dependent mechanism in adipose tissue. 5As mentioned above, Opsin 3 is extensively expressed in several brain regions, including cortex, hippocampus and hypothalamus. Opsin 3 expression in brain regions associated with circadian rhythms, such as the hypothalamus, may suggest a possible role in regulating these rhythms. This connection could influence sleep-wake cycles and other circadian-related functions. In addition, Opsin 3 expression in regions like the hippocampus, a key area for memory and learning, indicates it might play a role in neural development or synaptic plasticity. This could have implications for learning, memory, and other cognitive functions. 4,6Finally, Opsin 3 has been detected in several cells of the immune system including T-cells and macrophages, implying that immune responses could be potentially regulated through light-related mechanisms.2,7

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

OPN3, Encephalopsin, Panopsin

Short Description :

A Rabbit Polyclonal antibody to Opsin 3 (extracellular)

Long Description :

Anti-Opsin 3 (extracellular) Antibody (#AOR-023) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Opsin 3 from mouse, rat and human samples.

Negative Control :

BLP-OR023

Positive Control :

Synonyms :

OPN3, Encephalopsin, Panopsin

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

OPN3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage