Anti-OXGR1/GPR99 Antibody | Alomone Labs AOR-016 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-OXGR1/GPR99 Antibody

catalog :

AOR-016

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse kidney (lanes 1 and 4), rat kidney (lanes 2 and 5) (1:600) and rat testis (lanes 3 and 6) (1:200) lysates: - 1-3. Anti-OXGR1/GPR99 Antibody (#AOR-016).4-6. Anti-OXGR1/GPR99 Antibody, preincubated with OXGR1/GPR99 Blocking Peptide (#BLP-OR016).

image 2 :

Expression of OXGR1/GPR99 in mouse hippocampus and cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-OXGR1/GPR99 Antibody (#AOR-016), (1: 200), followed by goat anti-rabbit-AlexaFluor-488. A. GPR99 staining (green) in hippocampal dentate gyrus, is detected in the granule layer (G, vertical arrows) and in neurons of the hilus (H, horizontal arrows). B. GPR99 immunoreactivity (green) in cerebellum, is observed in neurons of the purkinje cell layer (P, vertical arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AOR-016

SKU :

AOR-016-CF_0.2 ml

Product Name :

Anti-OXGR1/GPR99 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6IYF8

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-OR016

Homology :

Rat - 12/14 amino acid residues identical; human - 9/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

CKASGDLEQGKKDS, corresponding to amino acid residues 319-332 of mouse OXGR1

Product Page - Scientific background :

Oxoglutarate receptor 1 (OXGR1) is a receptor for α-ketoglutarate (αKG), a metabolite that is a citric acid cycle intermediate and a glutamine catabolism by-product1. αKG also serves as a metabolic marker and its interaction with OXGR1 has been shown to mediate the adaptive regulation of HCO3- secretion and NaCl reabsorption under acid-base stress3.OXGR1 belongs to the G-protein coupled receptor (GPCRs) protein family. Members of this group contain seven trans-membrane domains and respond to a large variety of ligands and thereby affect key cellular functions that are crucial for cell survival or proliferation2. OXGR1 belongs to a subset of metabolic GPCRs, along with receptors for succinate (GPR91), lactate (GPR81), fatty acids (FFAR) and many more3.OXGR1 is expressed in the kidney3,4 and in the heart, where it affects the contractile function, consistent with the finding that α-ketoglutarate levels are high in heart failure patients5.Because of its major role in maintaining cellular balance and its role in heart contractile activity, identification of molecules that can specifically activate or inhibit this receptor may be very useful in the development of novel therapeutic approach for pathological cardiac hypertrophy5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Oxoglutarate receptor 1, 2-Oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, aKGR, GPR80, P2RY15, P2Y15

Short Description :

A Rabbit Polyclonal Antibody to Oxoglutarate Receptor 1

Long Description :

Anti-OXGR1/GPR99 Antibody (#AOR-016) is a highly specific antibody directed against an epitope of the mouse Oxoglutarate receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize OXGR1 from rat, mouse, and human samples.

Negative Control :

BLP-OR016

Positive Control :

Synonyms :

Oxoglutarate receptor 1, 2-Oxoglutarate receptor 1, Alpha-ketoglutarate receptor 1, aKGR, GPR80, P2RY15, P2Y15

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

OXGR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:600

more info or order :

Alomone Labs product webpage