Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody | Alomone Labs AOR-015-AR product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody

catalog :

AOR-015-AR

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 594

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Leo M, Schmitt L, Jastrow H, Thomale J, Kleinschnitz C, Hagenacker T. Cisplatin alters the function and expression of N-type voltage-gated calcium channels in the absence of morphological damage of sensory neurons. Mol Pain. 2017;13:1744806917746565 pubmed publisher

image

image 1 :

Expression of Nociceptin receptor in mouse hippocampus - Immunohistochemical staining of mouse hippocampus using Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody (#AOR-015-AR), (1:60). A, B. Nociceptin receptor staining (red) in hippocampal dentate gyrus (DG) appears in the outlines of neuronal cell bodies in the granule layer and in the hilus (arrows). C, D. Nociceptin receptor staining in hippocampal CA3 region appears in the outlines of neuronal cell bodies in the pyramidal layer (P). Nuclei are stained using DAPI as the counterstain.

image 2 :

Peptide (C)KQFKTSYEKRSFK corresponding to amino acids 188-200 of rat EAAT1 (AccessionP24942). 2ndextracellularloop.

product information

CAT :

AOR-015-AR

SKU :

AOR-015-AR_50 mcl

Product Name :

Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P35370

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-OR015

Homology :

Mouse - identical; human - 13/16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Intracellular, C-terminus

Label :

ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HREMQVSDRVRSIAKD, corresponding to amino acid residues 337-352 of rat OPRL1

Product Page - Scientific background :

Nociceptin/orphanin FQ (N/OFQ), a 17 amino acid peptide, is the endogenous agonist of the nociceptin/orphanin FQ peptide (NOP) receptor (previously termed ORL1 receptor) which belongs to the class A (Rhodopsin-like) γ subfamily of G protein-coupled receptors (GPCRs). Like all members the receptor has a common seven-transmembrane helical structure and is coupled predominantly to heterotrimeric Gi/Go proteins. N/OFQ receptor shows a high degree of structural homology with the μ-, δ- and κ-opioid receptors1. The intracellular events triggered by the binding of N/OFQ with the NOP receptor include inhibition of adenylyl cyclase, activation of phospholipase C (PLC) and of K+ channels, inhibition of Ca2+ channels, and activation of mitogen-activated protein-kinases (MAPKs). In neural tissues, the main effect is the inhibition of the release of neurotransmitters (acetylcholine, catecholamines, GABA, glutamate, 5-hydroxytryptamine, tachykinines)2.NOP receptor mRNA is detected in high levels in the cortex, anterior olfactory nucleus, lateral septum, hypothalamus, hippocampus, amygdala, central grey, pontine nuclei, interpeduncular nucleus, substantia nigra, raphe complex, locus coeruleus and spinal cord3.Studies with N/OFQ, NOP-selective agonists or antagonists, and receptor- or peptide-deficient mice have shown that the NOP system has important roles in the control of central and peripheral functions including pain, anxiety depression, hyperphagia and obesity, addiction, Parkinson's disease, cognition, food intake, learning and memory, locomotion, cough and micturition reflexes, cardiovascular homeostasis, intestinal motility and immune responses4.

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

κ-Type 3 opioid receptor, KOR-3, Orphanin FQ receptor, ROR-C, XOR1, ORL1, N/OFQ receptor

Short Description :

A Rabbit Polyclonal Antibody to Nociceptin Receptor Conjugated to the Fluorescent Dye ATTO-594

Long Description :

Anti-Nociceptin Receptor (OPRL1) Antibody (#AOR-015) is a highly specific antibody directed against an epitope the rat protein. The antibody can be used in western blot analysis, and has been designed to recognize the nociceptin receptor from mouse, rat and human samples. Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody (#AOR-015-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-Nociceptin Receptor (OPRL1)-ATTO Fluor-594 Antibody has been tested in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-OR015

Positive Control :

Synonyms :

κ-Type 3 opioid receptor, KOR-3, Orphanin FQ receptor, ROR-C, XOR1, ORL1, N/OFQ receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

OPRL1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #AOR-015), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:60

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage