Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody | Alomone Labs AOR-014 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody

catalog :

AOR-014

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Voskobiynyk Y, Battu G, Felker S, Cochran J, Newton M, Lambert L, et al. Aberrant regulation of a poison exon caused by a non-coding variant in a mouse model of Scn1a-associated epileptic encephalopathy. PLoS Genet. 2021;17:e1009195 pubmed publisher
Reyes B, Kravets J, Connelly K, Unterwald E, van Bockstaele E. Localization of the delta opioid receptor and corticotropin-releasing factor in the amygdalar complex: role in anxiety. Brain Struct Funct. 2017;222:1007-1026 pubmed publisher
Reed Geaghan E, Wright M, See L, Adelman P, Lee K, Koerber H, et al. Merkel Cell-Driven BDNF Signaling Specifies SAI Neuron Molecular and Electrophysiological Phenotypes. J Neurosci. 2016;36:4362-76 pubmed publisher

image

image 1 :

Western blot analysis of a rat cortex lysate: - 1. Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody (#AOR-014), (1:200).2. Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody, preincubated with δ-Opioid Receptor/OPRD1 (extracellular) Blocking Peptide (#BLP-OR014).

image 2 :

Expression of δ opioid receptor (DOR-1) in rat spinal cord - Immunohistochemical staining of rat spinal cord frozen section using Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody (#AOR-014), (1:100), followed by goat-anti-rabbit AlexaFluor-488 secondary antibody (green). Staining is present in neuronal cell bodies (white arrows). Hoechst 33342 is used as the counterstain (blue).

image 3 :

Expression of?opioid receptor (DOR-1) in rat spinal cord - Immunohistochemical staining of rat spinal cord frozen section usingAnti-?-Opioid Receptor (OPRD1) (extracellular) Antibody(#AOR-014) (1:100) followed by goat-anti-rabbit AlexaFluor-488 secondary antibody (green). Staining is present in neuronal cell bodies (white arrows). Hoechst 33342 is used as the counterstain (blue).

product information

CAT :

AOR-014

SKU :

AOR-014-CF_0.2 ml

Product Name :

Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P32300

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-OR014

Homology :

Rat - 13/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ELVPSARAELQSSPLVN corresponding to amino acid residues 2-18 of mouse δ-Opioid receptor

Product Page - Scientific background :

Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (µ)-opioid (MOP) receptor, delta (δ)-opioid (DOP) receptor, and kappa (κ)-opioid (KOP) receptor.1 Recently, the nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic µ, δ, and κ receptors.2The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins.1All three classic opioid receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the µ-opioid receptor, whereas µ-, δ-, and κ-receptors participate in the control of pain at the spinal level.3 The opioid receptors also mediate the mood-altering properties of opioids.4 Cross-talk between µ- and δ- opioid receptors was demonstrated when subeffective doses of d-opioid receptors agonists modulated m-mediated analgesia.5The δ receptors are discretely distributed in the central nervous system (CNS), with a prominent gradient of receptor density from high levels in forebrain structures to relatively low levels in most hindbrain regions.2

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

DOR-1

Short Description :

A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRD1)

Long Description :

Anti-δ-Opioid Receptor (OPRD1) (extracellular) Antibody (#AOR-014) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially detect the receptor in living cells. The antibody is designed to recognize DOR-1 from mouse and rat samples. It will not recognize DOR-1 from human samples.

Negative Control :

BLP-OR014

Positive Control :

Synonyms :

DOR-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

OPRD1

AB Specifictiy :

Will not recognize human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage