Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody | Alomone Labs AOR-012 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody

catalog :

AOR-012

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Wu Z, Wu S, Lee S, Lin C, Chang C, Lo J, et al. Dose-Dependent Effect of Hyperbaric Oxygen Treatment on Burn-Induced Neuropathic Pain in Rats. Int J Mol Sci. 2019;20: pubmed publisher

image

image 1 :

Western blot analysis of mouse brain (lanes 1 and 3), rat brain (lanes 2 and 4) and human brain neuroblastoma (SH-SY5Y) cell (lanes 5 and 6) lysates: - 1,2,5. Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody (#AOR-012), (1:200).3,4,6. Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody, preincubated with κ-Opioid Receptor/OPRK1 (extracellular) Blocking Peptide (#BLP-OR012).

image 2 :

Peptide (C)NGSVGSEDQQLEPAHIS corresponding to amino acid residues 39-55 of rat ?-Opioid receptor (AccessionP34975). Extracellular N-terminus.

image 3 :

Western blot analysis of human MCF-7 breast adenocarcinoma cell line lysate: - 1. Anti-Human TNF Receptor I (extracellular) Antibody(#ANT-041) (1:200).2. Anti-Human TNF Receptor I (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

AOR-012

SKU :

AOR-012-CF_0.2 ml

Product Name :

Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P34975

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-OR012

Homology :

Mouse -16/17 amino acid residues identical; human - 15/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)NGSVGSEDQQLEPAHIS, corresponding to amino acid residues 39-55 of rat κ-Opioid receptor

Product Page - Scientific background :

Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (µ)-opioid (MOP) receptor, delta (δ)-opioid (DOP) receptor, and kappa (κ)-opioid (KOP) receptor1. Recently, the nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic µ, δ, and κ receptors2.The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins1.All three classic opioid receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the µ-opioid receptor, whereas µ-, δ-, and κ-receptors participate in the control of pain at the spinal level3. The opioid receptors also mediate the mood-altering properties of opioids4.κ-Opioid Receptor is activated by the endogenous peptide dynorphin. Malfunction of their signaling may be involved in epilepsy, addiction, depression, schizophrenia, and chronic pain5.κ-Opioid Receptor is localized to a number of regions in the brain, as well as the spinal cord, the enteric nervous system and the digestive system5.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Kappa-type opioid receptor, KOR-1, Opiate receptor kappa 1

Short Description :

A Rabbit Polyclonal Antibody to ?-Opioid Receptor (OPRK1)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat κ-opioid receptor. Anti-κ-Opioid Receptor (OPRK1) (extracellular) Antibody (#AOR-012) can be used in western blot analysis. The antibody recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. The antibody was designed to recognize κ-opioid receptor from mouse, rat and human samples.

Negative Control :

BLP-OR012

Positive Control :

Synonyms :

Kappa-type opioid receptor, KOR-1, Opiate receptor kappa 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

OPRK1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage