Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody | Alomone Labs AOR-011 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody

catalog :

AOR-011

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Wu Z, Wu S, Lee S, Lin C, Chang C, Lo J, et al. Dose-Dependent Effect of Hyperbaric Oxygen Treatment on Burn-Induced Neuropathic Pain in Rats. Int J Mol Sci. 2019;20: pubmed publisher
Cleymaet A, Gallagher S, Tooker R, Lipin M, Renna J, Sodhi P, et al. μ-Opioid Receptor Activation Directly Modulates Intrinsically Photosensitive Retinal Ganglion Cells. Neuroscience. 2019;408:400-417 pubmed publisher
Ferrari L, Araldi D, Bogen O, Green P, Levine J. Systemic Morphine Produces Dose-dependent Nociceptor-mediated Biphasic Changes in Nociceptive Threshold and Neuroplasticity. Neuroscience. 2019;398:64-75 pubmed publisher
Leduc Pessah H, Weilinger N, Fan C, Burma N, Thompson R, Trang T. Site-Specific Regulation of P2X7 Receptor Function in Microglia Gates Morphine Analgesic Tolerance. J Neurosci. 2017;37:10154-10172 pubmed publisher

image

image 1 :

Western blot analysis of rat hippocampus lysate: - 1. Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody (#AOR-011), (1:200).2. Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody, preincubated with µ-Opioid Receptor/OPRM1 (extracellular) Blocking Peptide (#BLP-OR011).

image 2 :

Expression of µ-opioid receptor (MOR-1) in rat spinal cord - Immunohistochemical staining of rat spinal cord frozen section using Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody (#AOR-011), (1:100), followed by goat anti-rabbit AlexaFluor-488 secondary antibody (green). Staining is present in both neuronal cell bodies (white arrows) and their prolongations (red arrows). Hoechst 33342 is used as the counterstain (blue).

image 3 :

Cell surface detection of -Opioid Receptor in live intact mouse BV-2 microglia cells: - ___ Cells.___ Cells + goat-anti-rabbit-FITC.___ Cells +Anti--Opioid Receptor (OPRM1) (extracellular) Antibody(#AOR-011) (5 g) + goat-anti-rabbit-FITC.

product information

CAT :

AOR-011

SKU :

AOR-011_0.2 ml

Product Name :

Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P33535

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-OR011

Homology :

Mouse - identical; human - 15/17 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

0.8 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CSPAPGSWLNLSHVDGN, corresponding to amino acid residues 22-38 of the rat µ-Opioid receptor

Product Page - Scientific background :

Endogenous opiates such as endorphins, endomorphins, and enkephalins, as well as opiate drugs (including morphine) exert their effects by binding to opioid receptors. Three "classic" types of opioid receptors have been identified: mu (µ)-opioid (MOP) receptor, delta (δ)-opioid (DOP) receptor, and kappa (κ)-opioid (KOP) receptor. Recently, the nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor was also described. Despite its significant sequence homology, its pharmacological profile differs greatly from those of the classic µ, δ, and κ receptors.1The opioid receptors belong to the G protein-coupled receptor (GPCR) superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop important for binding G proteins.2All three receptors mediate opioid-induced analgesia. Supraspinal analgesia is mainly mediated by the µ-receptors, whereas µ-, δ-, and κ-receptors participate in the control of pain at the spinal level. These receptors also mediate the mood-altering properties of opioids.3Of the opioid receptors, the µ-opioid receptor has been the most extensively studied due to its important role in mediating the actions of morphine and other analgesic agents, as well as other addictive drugs such as heroin.1 The µ-opioid receptors are expressed in the central nervous system (CNS) and in the peripherial nervous system. The highest densities are found in the thalamus, caudate putamen, neocortex, amygdala, and other brain regions known to have well established roles in pain and analgesia.4

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Mu-type opioid receptor, Opioid receptor B, MOR-1, MOP, Mu-opiate receptor

Short Description :

A Rabbit Polyclonal Antibody to µ-Opioid Receptor (OPRM1)

Long Description :

Anti-µ-Opioid Receptor (OPRM1) (extracellular) Antibody (#AOR-011) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize MOR-1 from rat, human, and mouse samples.

Negative Control :

BLP-OR011

Positive Control :

Synonyms :

Mu-type opioid receptor, Opioid receptor B, MOR-1, MOP, Mu-opiate receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

OPRM1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage