Anti-Orexin Receptor 2 Antibody | Alomone Labs AOR-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Orexin Receptor 2 Antibody

catalog :

AOR-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Parekh R, White A, Leffler K, Biancardi V, Eells J, Abdel Rahman A, et al. Hypothalamic kinin B1 receptor mediates orexin system hyperactivity in neurogenic hypertension. Sci Rep. 2021;11:21050 pubmed publisher
Chamera K, Kotarska K, Szuster Głuszczak M, Trojan E, Skórkowska A, Pomierny B, et al. The prenatal challenge with lipopolysaccharide and polyinosinic:polycytidylic acid disrupts CX3CL1-CX3CR1 and CD200-CD200R signalling in the brains of male rat offspring: a link to schizophrenia-like behaviours. J Neuroinflammation. 2020;17:247 pubmed publisher
Zhang G, Wu X, Xu G, Weng S, Yang X, Zhong Y. Orexin-B modulates synaptic transmission of rod bipolar cells in rat retina. Neuropharmacology. 2018;133:38-50 pubmed publisher
Fan Y, Jiang E, Hahka T, Chen Q, Yan J, Shan Z. Orexin A increases sympathetic nerve activity through promoting expression of proinflammatory cytokines in Sprague Dawley rats. Acta Physiol (Oxf). 2018;222: pubmed publisher
Yokota S, Oka T, Asano H, Yasui Y. Orexinergic fibers are in contact with Kölliker-Fuse nucleus neurons projecting to the respiration-related nuclei in the medulla oblongata and spinal cord of the rat. Brain Res. 2016;1648:512-523 pubmed publisher

image

image 1 :

Western blot analysis of human Colo-205 (lanes 1 and 3) and HT-29 (lanes 2 and 4) colon cancer cell lysates: - 1,2. Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:200).3,4. Anti-Orexin Receptor 2 Antibody, preincubated with Orexin Receptor 2 Blocking Peptide (#BLP-OR002).

image 2 :

Expression of OX2R in rat brain - Immunohistochemical staining of rat brain using Anti-Orexin Receptor 2 Antibody (#AOR-002), (1:50), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (1:500), (red). A. In the cerebellum, staining is present in cell bodies of both Golgi type I (Purkinje cells – white arrows) and Golgi type II (green arrows) neurons. Staining is also present in fibers in the molecular layer (ML). B. In the parietal cortex, staining is evident in neural cell bodies. C. In the hippocampus, staining is present in pyramidal cells in the CA1 layer. Note that both cell bodies and prolongations are stained. Hoechst 33342 is used as the counterstain (blue).

image 3 :

Expression of OX2R in rat brain - Immunohistochemical staining of rat brain usingAnti-Orexin Receptor 2Antibody (#AOR-002) (1:50) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (1:500) (red). A.In the cerebellum staining is present in cell bodies of both Golgi type I (Purkinje cells ? white arrows) and Golgi type II (green arrows) neurons. Staining is also present in fibers in the molecular layer (ML). B.In the parietal cortex staining is evident in neural cell bodies. C.In the hippocampus staining is present in pyramidal cells in the CA1 layer. Note that both cell bodies and prolongations are stained.Hoechst 33342 is used asthe counterstain (blue).

product information

CAT :

AOR-002

SKU :

AOR-002-CF_0.2 ml

Product Name :

Anti-Orexin Receptor 2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P56719

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-OR002

Homology :

Mouse - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DRLARGRTSTESRKS, corresponding to amino acid residues 391-405 of rat OX2R

Product Page - Scientific background :

Orexin Receptor 2 (OX2R) (also known as hypocretin receptor 2) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX2R binds both orexin A and B with similar affinities while OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference).2,3Both OX2R and OX1R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. OX2R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels. OX2R can also transmit signals through inhibitory Gi G proteins, although the mechanism is less well understood.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research over the last few years. OX2R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2The best studied physiological role of OX2R is its involvement in the regulation of sleep and wakefulness states. Studies in mice lacking the orexin gene and dogs expressing a null mutation of the OX2R show a remarkably similar phenotype to human narcolepsy, a condition characterized by excessive daytime sleepiness, inability to maintain vigilant states, and defects in the regulation of rapid eye movement (REM) sleep.4In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

OX2R, Hypocretin receptor type 2, HCRTR2

Short Description :

A Rabbit Polyclonal Antibody to Orexin Receptor 2

Long Description :

Anti-Orexin Receptor 2 Antibody (#AOR-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX2R from rat, mouse, and human samples.

Negative Control :

BLP-OR002

Positive Control :

Synonyms :

OX2R, Hypocretin receptor type 2, HCRTR2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HCRTR2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage