Anti-Orexin Receptor 1 Antibody | Alomone Labs AOR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Orexin Receptor 1 Antibody

catalog :

AOR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Parekh R, White A, Leffler K, Biancardi V, Eells J, Abdel Rahman A, et al. Hypothalamic kinin B1 receptor mediates orexin system hyperactivity in neurogenic hypertension. Sci Rep. 2021;11:21050 pubmed publisher
Bigalke J, Gao H, Chen Q, Shan Z. Activation of Orexin 1 Receptors in the Paraventricular Nucleus Contributes to the Development of Deoxycorticosterone Acetate-Salt Hypertension Through Regulation of Vasopressin. Front Physiol. 2021;12:641331 pubmed publisher
Hammels I, Binczek E, Schmidt Soltau I, Jenke B, Thomas A, Vogel M, et al. Novel CB1-ligands maintain homeostasis of the endocannabinoid-system in ω3- and ω6-long chain-PUFA deficiency. J Lipid Res. 2019;: pubmed publisher
Zhang G, Wu X, Xu G, Weng S, Yang X, Zhong Y. Orexin-B modulates synaptic transmission of rod bipolar cells in rat retina. Neuropharmacology. 2018;133:38-50 pubmed publisher
Huber M, Fan Y, Jiang E, Zhu F, Larson R, Yan J, et al. Increased activity of the orexin system in the paraventricular nucleus contributes to salt-sensitive hypertension. Am J Physiol Heart Circ Physiol. 2017;313:H1075-H1086 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain lysate: - 1. Anti-Orexin Receptor 1 Antibody (#AOR-001), (1:200).2. Anti-Orexin Receptor 1 Antibody, preincubated with Orexin Receptor 1 Blocking Peptide (#BLP-OR001).

image 2 :

Expression of OX1R in rat brain - Longitudinal frozen section of rat brainstem showing staining (red) in neuronal cell bodies. Slides were incubated overnight at 4°C with Anti-Orexin Receptor 1 Antibody (#AOR-001), (1:50) followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (1:500). Hoechst 33342 is used as a counterstain (blue).

image 3 :

Western blot analysis of rat brain lysate: - 1.Anti-Orexin Receptor 1 Antibody (#AOR-001) (1:500).2. Anti-Orexin Receptor 1 Antibody preincubated with the control peptide antigen.

product information

CAT :

AOR-001

SKU :

AOR-001-CF_0.2 ml

Product Name :

Anti-Orexin Receptor 1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P56718

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-OR001

Homology :

Mouse - identical; human - 9/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R

Product Page - Scientific background :

Orexin Receptor 1 (OX1R) (also known as hypocretin receptor 1) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference), while OX2R binds both ligands with similar affinities.2,3Both OX1R and OX2R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.OX1R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research in the last few years.OX1R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2 It appears to be involved in the regulation of feeding behavior in rodents since an OX1R antagonist is able to inhibit baseline feeding.2,3The orexin system has been shown to be involved in the regulation of sleep and wakefulness states and OX1R knockout mice show defects in the regulation of rapid eye movement (REM) sleep, among other phenotypic alterations.4 In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

OX1R, Hypocretin receptor type 1, HCRTR1

Short Description :

A Rabbit Polyclonal Antibody to Orexin Receptor 1

Long Description :

Anti-Orexin Receptor 1 Antibody (#AOR-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX1R from rat, mouse, and human samples.

Negative Control :

BLP-OR001

Positive Control :

Synonyms :

OX1R, Hypocretin receptor type 1, HCRTR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HCRTR1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:100

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage