Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody | Alomone Labs AOR-001-AG product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody

catalog :

AOR-001-AG

clonality :

polyclonal

host :

domestic rabbit

conjugate :

ATTO 488

clone name :

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Parekh R, White A, Leffler K, Biancardi V, Eells J, Abdel Rahman A, et al. Hypothalamic kinin B1 receptor mediates orexin system hyperactivity in neurogenic hypertension. Sci Rep. 2021;11:21050 pubmed publisher
Bigalke J, Gao H, Chen Q, Shan Z. Activation of Orexin 1 Receptors in the Paraventricular Nucleus Contributes to the Development of Deoxycorticosterone Acetate-Salt Hypertension Through Regulation of Vasopressin. Front Physiol. 2021;12:641331 pubmed publisher

image

image 1 :

Multiplex staining of OX1R and Parathyroid hormone 1 receptor in rat ventromedial hypothalamus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody (#AOR-001-AG), (1:60) and Anti-PTH1R (extracellular) Antibody (#APR-051), (1:60). A. Sections were incubated with Anti-PTH1R (extracellular) Antibody, followed by donkey-anti-rabbit-Cy3 (red). B. The same sections were incubated with Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody, (green). C. Merge of A and B shows staining of OX1R in nerve fibers (vertical arrows) while Parathyroid hormone 1 receptor is apparent on cell bodies (horizontal arrows). Cell nuclei are stained with DAPI (blue).

image 2 :

Immuno-colocalization ofParathyroid hormone receptor 1andOrexin receptor 1in rat ventromedial hypothalamus - Immunohistochemical staining of perfusion-fixed frozen rat brain sectionsusingAnti-Orexin Receptor 1-ATTO-488 Antibody(#AOR-001-AG) (1:60) andAnti-PTH1R (extracellular) Antibody(#APR-051) (1:60). A. Sections were incubated with Anti-PTH1R (extracellular) Antibody followed by donkey-anti-rabbit-Cy3 (red). B. The same sections were incubated with Anti-Orexin Receptor 1-ATTO-488Antibody (green). C. Merge of A and B shows staining of Orexin receptor 1 in nerve fibers (vertical arrows) while Parathyroid hormone receptor 1 is apparent on cell bodies (horizontal arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

AOR-001-AG

SKU :

AOR-001-AG_50 mcl

Product Name :

Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P56718

Applications :

IF IHC

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-OR001

Homology :

Mouse - identical; human - 9/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

3rd intracellular loop

Label :

ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 - 515 nm range. This label is analogous to the dye fluorescein isothiocyanate (FITC) and can be used with filters used to detect FITC.

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R

Product Page - Scientific background :

Orexin Receptor 1 (OX1R) (also known as hypocretin receptor 1) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference), while OX2R binds both ligands with similar affinities.2,3Both OX1R and OX2R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.OX1R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research in the last few years.OX1R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2 It appears to be involved in the regulation of feeding behavior in rodents since an OX1R antagonist is able to inhibit baseline feeding.2,3The orexin system has been shown to be involved in the regulation of sleep and wakefulness states and OX1R knockout mice show defects in the regulation of rapid eye movement (REM) sleep, among other phenotypic alterations.4 In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4

Applications may also work in :

IF IHC

Supplier :

Alomone Labs

Target :

OX1R, Hypocretin receptor type 1, HCRTR1

Short Description :

A Rabbit Polyclonal Antibody to Orexin Receptor 1 Conjugated to the Fluorescent Dye ATTO-488

Long Description :

Anti-Orexin Receptor 1 Antibody (#AOR-001) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX1R from rat, mouse, and human samples. Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody (#AOR-001-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-Orexin Receptor 1-ATTO Fluor-488 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

Negative Control :

RIC-001-AG

Positive Control :

Synonyms :

OX1R, Hypocretin receptor type 1, HCRTR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

HCRTR1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody,#AOR-001), and immunohistochemistry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:60

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage