antibody

Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody

ANX-033

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

ANX-033-CF_0.2 ml

Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody

Antibodies

Antibodies

Polyclonal

P26433

WB

Human Mouse

Rabbit

BLP-NX033

Mouse - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Intracellular, C-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)EQREPKLNEKLHGR, corresponding to amino acid residues 466-479 of rat NHE-3

Maintaining physiological intracellular pH values is vital for most cells. The Na+/H+ exchanger family (NHE, also known as SLC9) is widely expressed and has a key role in protecting cells against acidification. There are currently six NHE transporters identified in mammalian cells1. The complete structure of members of the NHE family has not been completely solved. However, all members are organized in a similar manner with 11 or 12 membrane spanning domains, carrying out the Na+ and H+ exchange and a varying length intracellular C-terminal domain being involved in regulation of the exchange activity. The transport of Na+ and H+ in NHEs occurs in the center of the N-terminus via a two inverted funnel area that is made up of two transmembrane regions. NHE3 is expressed in sodium absorptive cells of the mammalian small intestine, colon, gall bladder, renal proximal tubule, and thick and thin limbs of the loop of Henle and can be mostly found in the plasma membrane2. NHE3 interacts with a multi-PDZ domain-containing scaffold protein called NHERF-1. NHERF-1 binds to the NHE3 C-terminus and has a role in NHE3 regulation in various cells. NHERF-1 functions as a cofactor required for the phosphorylation and downregulation of NHE3 activity in response to elevated intracellular cAMP3. In addition, silencing of NHERF-1 in a model of diabetic mice was found to prevent NHE3 trafficking and insulin-dependent activation4.

WB

Alomone Labs

Sodium/hydrogen exchanger 3, SLC9A3

A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 3 (NHE-3)

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NHE-3. Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody (#ANX-033) can be used in western blot analysis. It has been designed to recognize NHE-3 from human, mouse and rat samples.

BLP-NX033

NA

Sodium/hydrogen exchanger 3, SLC9A3

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SLC9A3

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:200