Anti-NCX3 (SLC8A3) Antibody | Alomone Labs ANX-013 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NCX3 (SLC8A3) Antibody

catalog :

ANX-013

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Liu Z, Cheng Q, Ma X, Song M. Suppressing Effect of Na+/Ca2+ Exchanger (NCX) Inhibitors on the Growth of Melanoma Cells. Int J Mol Sci. 2022;23: pubmed publisher
Piccialli I, Ciccone R, Secondo A, Boscia F, Tedeschi V, de Rosa V, et al. The Na+/Ca2+ Exchanger 3 Is Functionally Coupled With the NaV1.6 Voltage-Gated Channel and Promotes an Endoplasmic Reticulum Ca2+ Refilling in a Transgenic Model of Alzheimer's Disease. Front Pharmacol. 2021;12:775271 pubmed publisher
Hu H, Wang S, Wang Y, Liu Y, Feng X, Shen Y, et al. Blockade of the forward Na+ /Ca2+ exchanger suppresses the growth of glioblastoma cells through Ca2+ -mediated cell death. Br J Pharmacol. 2019;176:2691-2707 pubmed publisher
Liu Q, Sun B, Zhao J, Wang Q, An F, Hu X, et al. Increased Piezo1 channel activity in interstitial Cajal-like cells induces bladder hyperactivity by functionally interacting with NCX1 in rats with cyclophosphamide-induced cystitis. Exp Mol Med. 2018;50:60 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 4), mouse brain lysates (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6): - 1-3. Anti-NCX3 (SLC8A3) Antibody (#ANX-013), (1:200).4-6. Anti-NCX3 (SLC8A3) Antibody, preincubated with NCX3/SLC8A3 Blocking Peptide (#BLP-NX013).

image 2 :

Expression of NCX3 in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-NCX3 (SLC8A3) Antibody (#ANX-013), (1:100). A. NCX3 staining (green) is expressed mostly in molecular layer (Mol) interneurons (arrow). B. The same section, stained with Parvalbumin (Red). C. Merge of A and B demonstrates that NCX3 appears to be expressed in several GABAergic neurons in the molecular layer of which Parvalbumin positive cells are only one sub-group (see arrow). DAPI counterstain (blue) displays the layout of cerebellar layers.

image 3 :

Western blot analysis of rat brain lysates (lanes 1 and 4) mouse brain lysates (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell lysates (lanes 3 and 6): - 1-3.Anti-NCX3 (SLC8A3) Antibody (#ANX-013) (1:200).4-6. Anti-NCX3 (SLC8A3) Antibody preincubated with the negative control antigen.

product information

CAT :

ANX-013

SKU :

ANX-013-CF_0.2 ml

Product Name :

Anti-NCX3 (SLC8A3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P70549

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NX013

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)PLEGKEVDESRRE, corresponding to amino acid residues 303-315 of rat NCX3

Product Page - Scientific background :

Ca2+ has proven to be a universal signaling molecule in excitable and non-excitable cells. However, being that its intracellular concentration is 1000 times lower than that of the extracellular milieu, it is important for the cell to keep this ratio for proper function. NCX, a Na+/Ca2+ exchanger is responsible for most of the efflux of Ca2+ from the cell1-3. The NCX transporter is a member of the SLC8 family of solute carriers which in turn belong to the CaCA superfamily1,4,5.NCX-1 is one of three Na+/Ca2+ exchangers (NCX-1, NCX-2, NCX-3) leading to one Ca2+ movement across the plasma membrane in exchange of three Na+ influx. However, the transporter can reverse the direction of the transport if the concentrations of Na+ and Ca2+ change6. The transporter has nine transmembrane domains and intracellular N- and C-terminals. Between tansmembrane domains 5 and 6, the presence of an extra-long intracellular loop, termed the f-loop, is responsible for regulating the activity of NCX-1 via several different mechanisms like ion binding, phosphorylation, etc. The f-loop also has sites which undergo alternative splicing7.Of the three NCX expressed in mammalian cells, NCX-1 is the most widely expressed. Its expression is detected in the heart, brain, and kidney. NCX-1 undergoes alternative splicing in a tissue-dependent manner. The first splice region does not change the overall structure of the protein but rather enables the expression of the gene specific to the tissues which require the expression of the gene. The second splicing site leads to a number of proteins varying in length. NCX-2 expression is much more limited; it is expressed only in neurons. NCX-3 is expressed in skeletal muscle and in some regions of the brain and undergoes alternative splicing in a similar fashion to that of NCX-11,8.Due to their central role in modulating Ca2+ levels in the cell, NCX exchangers are involved in various pathophysiological diseases/disorders such as hypoxia, aging, Alzheimer's disease7, to name a few.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Na+/Ca2+ exchange protein 3, Sodium/calcium exchanger 3

Short Description :

A Rabbit Polyclonal Antibody to Na+/Ca2+ Exchanger 3 (SLC8A3)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat Na+/Ca2+ exchange protein 3. Anti-NCX3 (SLC8A3) Antibody (#ANX-013) can be used in western blot and immunohistochemistry applications. It has been designed to recognize NCX3 from human, rat and mouse samples.

Negative Control :

BLP-NX013

Positive Control :

Synonyms :

Na+/Ca2+ exchange protein 3, Sodium/calcium exchanger 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC8A3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage