Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody | Alomone Labs ANX-010 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody

catalog :

ANX-010

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Becskeh xe1 zi E, Kors xf3 s M, G xe1 l E, Tiszlavicz L, Hoyk Z, Deli M, et al. Inhibition of NHE-1 Increases Smoke-Induced Proliferative Activity of Barrett's Esophageal Cell Line. Int J Mol Sci. 2021;22: pubmed publisher
Mazzocchi M, Di Giusto G, Porta M, Pizzoni A, Beltramone N, Ford P, et al. Na+/H+ exchanger isoform 1 activity in AQP2-expressing cells can be either proliferative or anti-proliferative depending on extracellular pH. J Physiol Biochem. 2020;76:37-48 pubmed publisher
Xu J, Elkaddi N, García Blanco A, Spielman A, Bachmanov A, Chung H, et al. Arginyl dipeptides increase the frequency of NaCl-elicited responses via epithelial sodium channel alpha and delta subunits in cultured human fungiform taste papillae cells. Sci Rep. 2017;7:7483 pubmed publisher
Laczkó D, Rosztoczy A, Birkás K, Katona M, Rakonczay Z, Tiszlavicz L, et al. Role of ion transporters in the bile acid-induced esophageal injury. Am J Physiol Gastrointest Liver Physiol. 2016;311:G16-31 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 6), mouse brain lysate (lanes 2 and 7), human MCF-7 breast adenocarcinoma cells (lanes 3 and 8), human U-87 MG glioblastoma cells (lanes 4 and 9) and human THP-1 acute monocytic leukemia cells (lanes 5 and 10): - 1-5. Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010), (1:200).6-10. Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody, preincubated with Na+/H+ Exchanger 1/NHE-1 (extracellular) Blocking Peptide (#BLP-NX010).

image 2 :

Expression of NHE1 in mouse substantia nigra pars compacta - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. NHE1 immunoreactivity (green) appears in neuronal profiles (arrows). Cell nuclei are stained with DAPI (blue).

image 3 :

Cell surface detection of NHE1 inlive intact THP-1 (human acute monocytic leukemia cells) cell line: - ___Cells + goat anti-rabbit-DyLight-488.___Cells +Anti-Na+/H+Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010) (1:20) + goat-anti-rabbit-DyLight-488.

product information

CAT :

ANX-010

SKU :

ANX-010-CF_0.2 ml

Product Name :

Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P26431

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NX010

Homology :

Mouse - identical; human, pig, dog - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RERSIGDVTTAPSE, corresponding to amino acid residues 54-67 of rat NHE-1

Product Page - Scientific background :

In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via Na+/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1. To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1,3,4. Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ 1,5,6. K+ ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated1,5,8. NHE-1 expression is ubiquitous and may serve as a housekeeping gene9.

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Sodium/hydrogen exchanger 1, SLC9A1, APNH

Short Description :

A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 1 (NHE-1)

Long Description :

Anti-Na+/H+ Exchanger 1 (NHE-1) Antibody (#ANX-010) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize NHE-1 from human, rat, and mouse samples.

Negative Control :

BLP-NX010

Positive Control :

Synonyms :

Sodium/hydrogen exchanger 1, SLC9A1, APNH

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC9A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage