Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular)-FITC Antibody | Alomone Labs ANX-010-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular)-FITC Antibody

catalog :

ANX-010-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of NHE-1 by direct flow cytometry in live intact human THP-1 monocytic leukemia cell line:

image 2 :

Cell surface detection of NHE-1 by direct flow cytometry in live intact human MEG-01 megakaryoblast leukemia cell line:

image 3 :

Cell surface detection of NHE-1 by direct flow cytometry in live intact mouse BV-2 microglia cell line:

product information

CAT :

ANX-010-F

SKU :

ANX-010-F_15 mcl

Product Name :

Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P26431

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NX010

Homology :

Mouse - identical; human, pig, dog - 13/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

1st extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RERSIGDVTTAPSE, corresponding to amino acid residues 54-67 of rat NHE-1

Product Page - Scientific background :

In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via Na+/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1. To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1,3,4. Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ 1,5,6. K+ ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated1,5,8. NHE-1 expression is ubiquitous and may serve as a housekeeping gene9.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Sodium/hydrogen exchanger 1, SLC9A1, APNH

Short Description :

A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 1 (NHE-1) (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-Na+/H+ Exchanger 1 (NHE-1) Antibody (#ANX-010) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, live cell imaging, and indirect live cell flow cytometry applications. It has been designed to recognize NHE-1 from human, rat, and mouse samples. Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular)-FITC Antibody (#ANX-010-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Sodium/hydrogen exchanger 1, SLC9A1, APNH

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC9A1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANX-010), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage