Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody | Alomone Labs ANX-006 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody

catalog :

ANX-006

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membranes (lanes 1 and 3) and new born rat brain membranes (lanes 2 and 4): - 1,2. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200).3,4. Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody, preincubated with Na+/H+ Exchanger 6/NHE-6 Blocking Peptide (#BLP-NX006).

image 2 :

Expression of NHE-6 in rat hippocampus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. NHE-6 staining (green) in hippocampal dentate gyrus region, appears in neuronal soma outlines in the hilus (horizontal arrows) and the outer granule layer (vertical arrows). Cell nuclei were stained with DAPI (blue).

product information

CAT :

ANX-006

SKU :

ANX-006-CF_0.2 ml

Product Name :

Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q92581

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NX006

Homology :

Mouse, rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EHLGVPENERRTTK, corresponding to amino acid residues 515-528 of human NHE-6

Product Page - Scientific background :

Intracellular pH regulation in the brain is extremely important in both physiological and pathophysiological conditions. Changes in Intracellular pH often result in altered neuronal excitability. In mammalian cells, intracellular pH against acidosis is generally regulated by ion transporters located on the plasma membrane called sodium-hydrogen exchangers (Na+/H+ exchanger, NHE)1,2. In addition to their role in regulating pH, NHE transporters also function in a coupled mode to mediate recovery from decreased cell volume1.Several studies have suggested that Na+/H+ exchange is the predominant pH-regulatory mode in isolated nerve terminals. In addition, Na+/H+ exchange plays a role in regulating synaptic transmission at glutamatergic, GABAergic, and dopaminergic synapses1.The NHE family belongs to the Slc9 gene family of Na+ coupled transport proteins and comprises nine mammalian isoforms of Na+/H+ exchangers that are localized to the plasma membrane, NHE1-9 encoded by the SLC9A1-SLC9A9 genes, respectively. SLC9 family members have a short N terminus and an extensive C terminus that plays a regulatory role2.NHE6 is localized to early and recycling endosomes, where it exchanges luminal H+ for Na+ or K+. Its expression is highest in the spinal cord and brain and is particularly enriched in the hippocampus, cortex, and Purkinje cell layer of the cerebellum3. Mutations in NHE6 encoding gene have been linked to autism, Christianson syndrome, and Angelman-like syndrome.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Sodium/hydrogen exchanger 6, NHE6, SLC9A6

Short Description :

A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 6 (NHE-6)

Long Description :

Anti-Na+/H+ Exchanger 6 (NHE-6) Antibody (#ANX-006) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NHE-6 from human, rat, and mouse samples.

Negative Control :

BLP-NX006

Positive Control :

Synonyms :

Sodium/hydrogen exchanger 6, NHE6, SLC9A6

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC9A6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage