antibody

Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody

ANX-005

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

ANX-005-CF_0.2 ml

Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody

Antibodies

Antibodies

Polyclonal

Q9Z0X2

WB

Human Rat Mouse

Rabbit

BLP-NX005

Mouse, human - identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Intracellular, C-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

CAEKELPWKSGQGD, corresponding to amino acid residues 759-772 of rat NHE-5

Intracellular pH regulation in the brain is extremely important under both physiological and physiopathological conditions. Changes in intracellular pH often result in altered neuronal excitability. In mammalian cells, intracellular pH against acidosis is generally regulated by ion transporters located in the plasma membrane called sodium-hydrogen exchangers (Na+/H+ exchanger, NHE)1. Several studies have suggested that Na+/H+ exchange is the predominant pH-regulatory mode in isolated nerve terminals. Na+/H+ exchanger also has a role in the regulation of synaptic transmission at glutamatergic, GABAergic, and dopaminergic synapses. In addition, NHE transporters also function in a coupled mode to mediate recovery from decreased cell volume1. The NHE family belongs to the Slc9 gene family of Na+ coupled transport proteins and comprises nine mammalian isoforms of Na+/H+ exchangers that are localized to the plasma membrane. NHE1-9 are encoded by the SLC9A1-SLC9A9 genes, respectively. The structure of SLC9 family members includes a short N-terminus and an extensive C-terminus that plays a regulatory role. NHE-5 exchanger corrects intracellular acidosis by exporting H+ in exchange for extracellular Na+, thereby tending to raise intracellular pH. Several studies have suggested that NHE5 plays a critical role in the synaptic pH regulation during the firing of action potentials1. It is predominantly detected in the brain with high expression in multiple regions such as the hippocampus and cerebral cortex3. NHE5 knockdown or overexpression of a dominant-negative mutation of NHE5 in cultured hippocampal neurons causes dendritic spine overgrowth. Therefore it has been suggested that NHE5 acts as a negative regulator of activity-dependent spine growth by regulating pH-sensitive synaptic proteins2,3.

WB

Alomone Labs

Sodium/hydrogen exchanger 5, SLC9A5

A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 5 (NHE-5)

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NHE-5. Anti-Na+/H+ Exchanger 5 (NHE-5) Antibody (#ANX-005) can be used in western blot analysis. It has been designed to recognize NHE-5 from human, rat and mouse samples.

BLP-NX005

NA

Sodium/hydrogen exchanger 5, SLC9A5

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SLC9A5

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:200