Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody | Alomone Labs ANX-002 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody

catalog :

ANX-002

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Yang L, Xu S, Guo X, Uchida S, Weinstein A, Wang T, et al. Regulation of renal Na transporters in response to dietary K. Am J Physiol Renal Physiol. 2018;315:F1032-F1041 pubmed publisher
Laczkó D, Rosztoczy A, Birkás K, Katona M, Rakonczay Z, Tiszlavicz L, et al. Role of ion transporters in the bile acid-induced esophageal injury. Am J Physiol Gastrointest Liver Physiol. 2016;311:G16-31 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney membranes (lanes 1 and 4), mouse kidney lysates (lanes 2 and 5) and rat stomach lysates (lanes 3 and 6): - 1-3. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:200).4-6. Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody, preincubated with Na+/H+ Exchanger 2/NHE-2 Blocking Peptide (#BLP-NX002).

image 2 :

Expression of Na+/H+ exchanger 2 in rat colon - Immunohistochemical staining of rat colon sections (paraffin-embedded) using Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002), (1:50). A. NHE-2 staining is shown in red. B. Cell nuclei were labeled with DAPI (blue). C. Merge of the two images.

product information

CAT :

ANX-002

SKU :

ANX-002-CF_0.2 ml

Product Name :

Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P48763

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NX002

Homology :

Mouse - identical; human - 12/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RASEPGNRKGRLGNEK, corresponding to amino acid residues 797-812 of rat NHE-2

Product Page - Scientific background :

In order to function in optimal conditions, cells must maintain a close to neutral intracellular pH. They have adopted various mechanisms in order to do so, one of which is via Na+/H+ exchangers (NHEs). Genes belonging to this group are expressed along a very broad range of organisms and are essential for protecting cells against intracellular acidification1.To date, nine genes have been identified in mammals; NHE1-9. These membrane proteins have 10-12 transmembrane domains depending on whether a splice variant is expressed and an intracellular N-terminal. The C-terminal domain can be either intracellular or extracellular, also depending whether a splice variant of the protein is involved. The C-terminal part of the protein also undergoes posttranslational modification such as phosphorylation2. Both NHE-1 and NHE-2 have an extracellular loop which is glycosylated1,3,4.Under physiological conditions, the Na+/H+ exchanger mediates the exchange of one extracellular Na+ ion for one intracellular proton, thereby keeping the overall charge neutral1. The extracellular binding site of Na+ is not selective as it can also bind Li+ and H+ 1,5,6. K+ ions inhibit NHE-1 but have no effect on NHE-27. The activation of NHE-1 and NHE-2 is sensitive to intracellular acidic pH. Under physiological conditions, both exchangers are not active and upon a drop of intracellular pH, they are rapidly activated1,5,8.NHE-2 is detected in the intestine, kidney and parietal cells2,9-11. It is also detected in skeletal muscle and testis2,12.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Sodium/hydrogen exchanger 2, SLC9A2

Short Description :

A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 2 (NHE-2)

Long Description :

Anti-Na+/H+ Exchanger 2 (NHE-2) Antibody (#ANX-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NHE-2 from human, rat, and mouse samples.

Negative Control :

BLP-NX002

Positive Control :

Synonyms :

Sodium/hydrogen exchanger 2, SLC9A2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC9A2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage