Anti-SLC11A1/NRAMP1 (extracellular) Antibody | Alomone Labs ANT-201 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC11A1/NRAMP1 (extracellular) Antibody

catalog :

ANT-201

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat spleen lysates (lanes 1 and 4), rat lung membranes (lanes 2 and 4) and mouse lung membranes (lanes 3 and 6): - 1-3. Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (1:200). 4-6. Anti-SLC11A1/NRAMP1 (extracellular) Antibody, preincubated with SLC11A1/NRAMP1 (extracellular) Blocking Peptide (#BLP-NT201) (#BLP-NT201).

image 2 :

Expression of NRAMP1 in rat hippocampus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201), (1:600), followed by goat anti-rabbit-Alexa-488. A. Staining in the dentate gyrus region showed NRAMP1 immunoreactivity (green) in the granule layer (G) and in hilar interneurons (arrows). B. Pre-incubation of the antibody with SLC11A1/NRAMP1 (extracellular) Blocking Peptide (#BLP-NT201), suppressed staining. Cell nuclei are stained with DAPI (blue). H = hilus.

product information

CAT :

ANT-201

SKU :

ANT-201-CF_0.2 ml

Product Name :

Anti-SLC11A1/NRAMP1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49279

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT201

Homology :

Human, rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

4th extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

CANSSLHDYAKIFP(R), corresponding to amino acid residues 322-335 of human SLC11A1

Product Page - Scientific background :

Solute carrier family 11 member a1 (Slc11a1) also known as natural resistance-associated macrophage protein 1 (NRAMP1) is a proton/divalent cation antiporter, known for its role in regulating the antimicrobial activity of macrophages1. The structure of Slc11a1 includes a common and conserved hydrophobic core of 10 transmembrane domains (TM) and either one or two non-conserved, highly hydrophobic TM domains4.Slc11a1 delivers divalent cations such as Fe2+, Zn2+, and Mn2+ from the cytosol to the late endosome/lysosome acidic compartments where the Fenton reaction can use ferrous iron to generate toxic hydroxyl radicals2. Thus, providing one mechanism by which Slc11a1 could influence antimicrobial activity in macrophages.Transition of metal ions is essential and participates in numerous cellular functions such as regulation of transcription. In addition, hundreds of enzymes require metal ions as co-factors3. Studies show that polymorphism in SLC11A1 is correlated to the occurrence of autoimmune and infectious disease susceptibility, as well as innate and adaptive immune responses to mycobacterial products/antigens. Therefore, research on this ion transporter is of high importance1.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1

Short Description :

A Rabbit Polyclonal Antibody to SLC11A1/NRAMP1

Long Description :

Anti-SLC11A1/NRAMP1 (extracellular) Antibody (#ANT-201) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize NRAMP1 from rat, mouse, and human samples.

Negative Control :

BLP-NT201

Positive Control :

Synonyms :

Natural Resistance-Associated Macrophage Protein 1, LSH, Solute Carrier Family 11 Member 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC11A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage