Anti-SLC35G1 (extracellular) Antibody | Alomone Labs ANT-196 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC35G1 (extracellular) Antibody

catalog :

ANT-196

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 3) and mouse brain membrane (lanes 2 and 4): - 1, 2. Anti-SLC35G1 (extracellular) Antibody (#ANT-196), (1:200). 3, 4. Anti-SLC35G1 (extracellular) Antibody, preincubated with SLC35G1 (extracellular) Blocking Peptide (#BLP-NT196).

product information

CAT :

ANT-196

SKU :

ANT-196-CF_0.2 ml

Product Name :

Anti-SLC35G1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q2M3R5

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT196

Homology :

Mouse - 12/15 amino acid residues identical; rat - 11/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

C)SDTSGMEESYSGHLK, corresponding to amino acid residues 209-223 of human SLC35G1

Product Page - Scientific background :

SLC35G1 (Solute carrier family 35 member G1) is a 10 transmembrane-spanning protein that interacts with STIM1 and is involved in calcium level regulation in the ER, plasma membrane and mitochondria1.Stromal interaction molecule 1 (STIM1) translocates within the ER membrane upon calcium depletion and interacts with Orai1 on the plasma membrane. STIM1 gates Orai1, allowing calcium to enter the cytoplasm, where it repletes the ER store via Calcium-ATPase pumps.SLC35G1 is located on the plasma membrane and ER. Upon calcium depletion, SLC35G1 binds STIM1 and moves it within the ER to localize it near the cell membrane. hence its role in regulating cellular calcium levels1,2.Due to its involvement in the STIM1-Orai1 axis, SLC35G1 mutations may have a role in immunodeficiency, muscular hypotonia, Stormorken syndrome and York platelet syndrome and many other calcium flux related syndromes3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Solute carrier family 35 member G1, Partner of STIM1, POST, Transmembrane protein 20, C10orf60, TMEM20

Short Description :

A Rabbit Polyclonal Antibody to SLC35G1 Transporter

Long Description :

Anti-SLC35G1 (extracellular) Antibody (#ANT-196) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize SLC35G1 from human, rat, and mouse samples.

Negative Control :

BLP-NT196

Positive Control :

Synonyms :

Solute carrier family 35 member G1, Partner of STIM1, POST, Transmembrane protein 20, C10orf60, TMEM20

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC35G1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage