Anti-SLC3A2 (CD98) (extracellular) Antibody | Alomone Labs ANT-192 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC3A2 (CD98) (extracellular) Antibody

catalog :

ANT-192

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysate (lanes 2 and 4):

image 2 :

Western blot analysis of mouse liver membranes (lanes 1 and 3) and mouse kidney lysate (lanes 2 and 4):

image 3 :

Western blot analysis of human U-87 MG glioma cell line lysate (lanes 1 and 3) and human HUVEC endothelial cell line lysate (lanes 2 and 4):

product information

CAT :

ANT-192

SKU :

ANT-192-CF_0.2 ml

Product Name :

Anti-SLC3A2 (CD98) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P10852

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT192

Homology :

Rat - identical Human - 14 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, C-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KGLVLGPIHKNQKDE, corresponding to amino acid residues 150 - 164 of mouse SLC3A2

Product Page - Scientific background :

Solute carrier family 3 member 2 (SLC3A2), also known as 4F2 cell-surface antigen heavy chain (4F2HC) and CD98 antigen heavy chain (CD98hc), is a type II membrane protein belonging to the SLC3A/CD98 transporter family that plays a role in various physiological processes including amino acid transport, cell differentiation, migration, and proliferation.1,2,4SLC3A2 is a type II glycosylated protein composed of an extracellular domain, a transmembrane domain, and a cytoplasmic domain. SLC3A2 forms obligatory heterodimeric complexes with six members of the L-type amino acid transporters (LATs) SLC7A5-11, and SLC7A13.1The heteromeric complex between SLC3A2 and SLC7A5 (also known as LAT1) is one of the best characterized. SLC7A5 is responsible for the transport of large neutral amino acids, including leucine, across the cell membrane. SLC3A2 acts as a chaperone protein for SLC7A5, stabilizing its expression on the cell surface and assisting in its proper functioning. This partnership is essential for amino acid transport, cell growth, and protein synthesis. This complex is particularly important in cancer cells and immune cells, as it facilitates nutrient uptake and influences cell proliferation and immune responses.1-4In addition to SLC7A5, SLC3A2 also forms a complex with another SLC7 family member called SLC7A11 (also known as xCT). This complex is commonly referred to as the CD98/xCT or SLC3A2/SLC7A11.1-4xCT, is a cystine/glutamate antiporter that plays a crucial role in the cellular exchange of cystine (an amino acid involved in the synthesis of the antioxidant glutathione) for glutamate. This exchange is important for maintaining cellular redox balance and protecting cells from oxidative stress.1,3,5When SLC3A2 partners with SLC7A11, it facilitates the function of the xCT antiporter, allowing cells to import cystine while exporting glutamate. This process is essential for antioxidant defence and protecting cells from damage caused by reactive oxygen species (ROS). The SLC3A2/SLC7A11 complex has significant implications in cancer biology and the modulation of immune responses, as it can influence cellular redox status and affect processes related to cell proliferation and immune cell function.1,3,5

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Solute Carrier Family 3 Member 2, 4F2 Cell-Surface Antigen Heavy Chain, 4F2HC, CD98 Antigen Heavy Chain, CD98HC

Short Description :

A Rabbit Polyclonal antibody to CALHM2 (extracellular)

Long Description :

Anti-SLC3A2 (CD98) (extracellular) Antibody (#ANT-192) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize SLC3A2 from mouse, rat and human samples.

Negative Control :

BLP-NT192

Positive Control :

Synonyms :

Solute Carrier Family 3 Member 2, 4F2 Cell-Surface Antigen Heavy Chain, 4F2HC, CD98 Antigen Heavy Chain, CD98HC

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC3A2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage