Anti-SLC38A9 Antibody | Alomone Labs ANT-189 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC38A9 Antibody

catalog :

ANT-189

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1-2. Anti-SLC38A9 Antibody (#ANT-189), (1:200). 3-4. Anti-SLC38A9 Antibody, preincubated with SLC38A9 Blocking Peptide (#BLP-NT189).

image 2 :

Expression of SLC38A9 in mouse cingulate cortex. - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections Anti-SLC38A9 Antibody (#ANT-189), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SLC38A9 immunoreactivity (green) appears in neurons (arrows). B. Pre-incubation of the antibody with SLC38A9 Blocking Peptide (#BLP-NT189), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANT-189

SKU :

ANT-189-CF_0.2 ml

Product Name :

Anti-SLC38A9 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8BGD6

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT189

Homology :

Rat, Human - 14 out of 15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-term.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)GSAYKLKSYTEGYGK, corresponding to amino acid residues 101 - 115 of mouse SLC38A9

Product Page - Scientific background :

Sodium-coupled neutral amino acid transporter 9 (SLC38A9) is a lysosomal amino acid transporter, involved in the regulation of mechanistic target of rapamycin complex 1 (mTORC1) activity in response to amino acid levels within the lysosomal lumen1,2. A sodium-couple amino acid transporter at the lysosome, SLC38A9, senses arginine from within the lysosome to convey arginine sufficiency to mTORC1.SLC38A9 forms a complex with other lysosomal resident proteins including the v-ATPase and a pentameric complex called Ragulator3-5. v-ATPase function is necessary for mTORC1 activation, but the molecular mechanism of this regulation is currently unclear2. Ragulator binds strongly to the Rag GTPases and was shown to function as a guanine exchange factor (GEF) for RagA/B6,7. Potentially SLC38A9 and the v-ATPase are able to regulate mTORC1 activity by modulating Ragulator's GEF activity. RagA/B is not the only Rag GTPase that is regulated, but a Folliculin-FNIP2 complex is a GAP for RagC/D8,9.In short, SLC38A9 is a functional component of the lysosomal amino acid sensing machinery involved in the control of mTORC1 activity, that underlies the regulation of the metabolic status and cellular responses to growth factors, energy, glucose and amino acid levels 4.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Sodium-Coupled Neutral Amino Acid Transporter 9, Solute Carrier Family 38 Member 9, Up-Regulated In Lung Cancer 11, URLC11

Short Description :

A Rabbit Polyclonal antibody to SLC38A9

Long Description :

Anti-SLC38A9 Antibody (#ANT-189) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC38A9 from rat, mouse and human samples.

Negative Control :

BLP-NT189

Positive Control :

Synonyms :

Sodium-Coupled Neutral Amino Acid Transporter 9, Solute Carrier Family 38 Member 9, Up-Regulated In Lung Cancer 11, URLC11

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC38A9

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage