Anti-SLC38A1 Antibody | Alomone Labs ANT-184 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC38A1 Antibody

catalog :

ANT-184

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3), rat placenta (lanes 2 and 4), (1:800), mouse brain membrane (lanes 5 and 6), (1:200), human U-87 MG (lanes 7 and 9), and human Jurkat (lanes 8 and 10), (1:500) cell lysates: - 1, 2, 5, 7, 8. Anti-SLC38A1 Antibody (#ANT-184). 3, 4, 6, 9, 10. Anti-SLC38A1 Antibody, preincubated with SLC38A1 Blocking Peptide (#BLP-NT184).

image 2 :

Expression of SLC38A1 in rat cerebellum. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC38A1 Antibody (#ANT-184), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SLC38A1 immunoreactivity (green) appears in neurons of the purkinje cell layer (P, vertical arrows) and in dendrites in the molecular layer (MOL, horizontal arrows). B. Pre-incubation of the antibody with SLC38A1 Blocking Peptide (BLP-NT184), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANT-184

SKU :

ANT-184-CF_0.2 ml

Product Name :

Anti-SLC38A1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JM15

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT184

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SKFISDRESRRSLTNSH, corresponding to amino acid residues 41-57 of rat SLC38A1

Product Page - Scientific background :

SNAT1 (SLC38A1, Sodium-coupled neutral amino acid transporter 1) is a 486 amino acids system A type sodium symporter that catalyzes the electrogenic Na+-dependent uptake of glutamine, among other substrates. SNAT1 is thought to be responsible for glutamine uptake into neurons1.SNAT1 belongs to the β-family of solute carriers that includes three SLC families, SLC32, SLC36, and SLC38. SNAT1 is a member of the SLC38 family that includes 11 members, SNAT1 to SNAT11.SNAT1 and SNAT2 are expressed in neurons and in glial cells all over the central nervous system, with high expression in perivascular glial profiles and lower expression in astrocytes of the cerebral parenchyma. This supports a role in controlling the amino acid permeability at the blood-brain barrier2. SNAT1 also acts as a positive regulator of mTORC1 signaling pathway in neurons and plays an important pathological role in the mechanism underlying neuronal survival after ischemia3. It was also shown to be up-regulated in specific types of cancer4.The structure of SNAT proteins is predicted to have a 5+5 inverted repeat fold typical of the bacterial LeuT, with intracellular N-terminus and extracellular and C-terminal tail5.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Sodium-coupled neutral amino acid transporter 1, Snat1, Amino acid transporter A1, rATA1, Glutamine transporter, N-system amino acid transporter 2, Ata1, Glnt, Sa2, Sat1

Short Description :

A Rabbit Polyclonal Antibody to SLC38A1 Transporter

Long Description :

Anti-SLC38A1 Antibody (#ANT-184) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize SNAT1 from human, rat, and mouse samples.

Negative Control :

BLP-NT184

Positive Control :

Synonyms :

Sodium-coupled neutral amino acid transporter 1, Snat1, Amino acid transporter A1, rATA1, Glutamine transporter, N-system amino acid transporter 2, Ata1, Glnt, Sa2, Sat1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC38A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage