antibody

Anti-LETM1 Antibody

ANT-183

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

ANT-183-CF_0.2 ml

Anti-LETM1 Antibody

Antibodies

Antibodies

Polyclonal

Q5XIN6

WB

Human Rat Mouse

Rabbit

BLP-NT183

Mouse -13/14 amino acid residues identical; human - 12/14 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Intracellular, mitochondrial matrix.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)KELSKTGDEKYIEE, corresponding to amino acid residues 587-600 of rat LETM1

LETM1 (leucine zipper-EF hand-containing transmembrane region), a protein with a key role in mitochondrial volume homeostasis through regulation of K+/H+ exchange (KHE), is involved in respiratory chain biogenesis and in the pathogenesis of seizures in the Wolf-Hirschhorn syndrome (WHS). LETM1 has been recently proposed to catalyze mitochondrial H+/Ca2+ exchange, which would imply a role in mitochondrial Ca2+ homeostasis as well.LETM1 contains a hydrophobic N-terminal portion spanning the inner membrane and a large hydrophilic portion including the C-terminus located in the matrix. The primary amino acid sequence of the C-terminal part predicts at least two coiled-coil domains and two Ca2+-binding EF hand-like motifs. However, the EF hands differ from canonical EF hand motifs by several residues, and the α helices are not directly flanking the Ca2+-binding loop. The amino acid sequence of yeast LETM1 proteins lacks these putative Ca2+-binding EF hand motifs. The N-terminal region of the LETM1 protein superfamily displays a mitochondrial targeting sequence and a single transmembrane domain. The latter is highly conserved in all orthologues and is rich in proline residues.In mitochondria, absence of LETM1, causes decompensation of electrophoretic K+, resulting in matrix swelling causing, in turn, increased mitophagy and/or cell death1.

WB

Alomone Labs

Leucine zipper-EF-hand-containing transmembrane protein 1, LETM1 and EF-hand domain-containing protein 1 mitochondrial, Mitochondrial proton/calcium exchanger protein, Mdm38 homolog

A Rabbit Polyclonal Antibody to LETM1

Anti-LETM1 Antibody (#ANT-183) is a highly selective antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize LETM1 from human, mouse, and rat samples.

BLP-NT183

NA

Leucine zipper-EF-hand-containing transmembrane protein 1, LETM1 and EF-hand domain-containing protein 1 mitochondrial, Mitochondrial proton/calcium exchanger protein, Mdm38 homolog

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

LETM1

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:1500