Anti-MRS2 Antibody | Alomone Labs ANT-148 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-MRS2 Antibody

catalog :

ANT-148

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat testis (lanes 1 and 4) and kidney lysates (lanes 2 and 5) and heart membrane (lanes 3 and 6): - 1-3. Anti-MRS2 Antibody (#ANT-148), (1:400).4-6. Anti-MRS2 Antibody, preincubated with MRS2 Blocking Peptide (#BLP-NT148).

product information

CAT :

ANT-148

SKU :

ANT-148-CF_0.2 ml

Product Name :

Anti-MRS2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9ET09

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT148

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

N-terminus, mitochondrial matrix.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DPKHSSVDRSKLH, corresponding to amino acid residues 211-223 of rat MRS2 magnesium transporter

Product Page - Scientific background :

Magnesium is one of the most abundant cation in the cell. It is crucial for the proper functioning of various tissues such as the brain, muscle, bone and the nervous system. Currently there are only two types of Mg2+ transport systems which have had their complete structures discovered. MgtE and CorA are the prokaryotic homologues of the SLC41 and MRS2 magnesium transporters.In eukaryotes, MRS2 protein is expressed in mitochondrial inner membranes. MRS2 is required for normal mitochondrial magnesium homeostasis and for proper myelination in the central nervous system. Notably, the expression of MRS2 serves as a genetic hallmark for embryonic stem cells2.MRS2 is comprised of two adjacent transmembrane domains. The first domain ends with a gly-met-asn motif and additional secondary structures. A long N-terminal and a short C-terminal sequence are located in the inner side of the mitochondrial membrane leaving the short loop connecting the transmembrane domains protruding to its exterior.In addition to its Mg2+ permeability, MRS2 transporters somewhat transport Ni2+. However, they are not permeable to Ca2+, Mn2+ and Co2+. MRS2 transporters can be blocked by Cobalt(III)-hexaamine, an analog of the hydrated Mg2+ ion and their activity is regulated by matrix Mg2+ concentrations. Increase in Mg2+ concentration causes a shift of the current-voltage relationship to a more negative potential as expected from the reduction in driving force and significantly reduces the channels' open-time probability2.Several SNPs in the MRS2 gene are associated an increased risk of developing type 2 diabetes in women of African American and Hispanic American origin3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

MRS2 magnesium transporter, Magnesium transporter MRS2 homolog mitochondrial, MRS2-like protein, MRS2L

Short Description :

A Rabbit Polyclonal Antibody to MRS2 Magnesium Transporter

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat MRS2 magnesium transporter. Anti-MRS2 Antibody (#ANT-148) can be used in western blot analysis. It has been designed to recognize MRS2 from mouse, rat and human samples.

Negative Control :

BLP-NT148

Positive Control :

Synonyms :

MRS2 magnesium transporter, Magnesium transporter MRS2 homolog mitochondrial, MRS2-like protein, MRS2L

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

MRS2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage