Anti-NIPAL4 (extracellular) Antibody | Alomone Labs ANT-144 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NIPAL4 (extracellular) Antibody

catalog :

ANT-144

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human acute monocytic leukemia cell line THP-1 (lanes 1 and 2), mouse B cell lymphoma cell line WEHI-231 (lanes 3 and 4), rat brain membranes (lanes 5 and 7) and mouse brain membranes (lanes 6 and 8): - 1,3,5,7. Anti-NIPAL4 (extracellular) Antibody (#ANT-144), (1:400).2,4,6,8. Anti-NIPAL4 (extracellular) Antibody, preincubated with NIPAL4 (extracellular) Blocking Peptide (#BLP-NT144).

product information

CAT :

ANT-144

SKU :

ANT-144-CF_0.2 ml

Product Name :

Anti-NIPAL4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q0D2K0

Applications :

IFC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT144

Homology :

Mouse - 13/15 amino acid residues identical; rat - 12/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)EKVTTIMEMASKMKD, corresponding to amino acid residues 242-256 of human magnesium transporter NIPA4

Product Page - Scientific background :

The NIPA family is currently comprised of 4 members- NIPA1-4. These members have a molecular similarity of 40%. NIPA proteins are integral membrane proteins which function as Mg2+ transporters.The NIPA4 gene in mice is located on chromosome 11B1.1 and the human corresponding gene, hIchthyin is located on chromosome 5q33.3. Both genes share an 85% amino acid similarity. Interestingly, NIPA2-4 show greater common identity in their ancestral invertebrate polypeptides than they do to NIPA1 suggesting different physiological roles. NIPA4 mediates Mg2+ uptake with a Km of 0.36 mM but unlike NIPA2, which is selective for Mg2+, it has been found to transport other cations as well.Mutations in the hIchthyin gene provide the basis of one form of autosomal-recessive congenital ichthyosis (ARCI). ARCI is a heterogenous group of disorders with abnormal differentiation and desquamation of the epidermis, leading to widespread scaling and erythema of the skin. To this day six homozygous mutations have been found, including one nonsense and five missense mutations, in a large number of consanguineous families presenting with ichthyosis. The function of ichthyin in skin differentiation is not fully understood but it has been suggested that because lipid dysfunctions are involved with the other ARCI disorders ichthyin might perform as a membrane receptor for trioxilins of the hepoxilin pathway.Although some NIPA proteins have been implicated as candidates for prader-willi syndrome their involvement remains unclear. Currently there is no evidence that NIPA4 is directly involved in the many complexities of the syndrome1.

Applications may also work in :

IFC WB

Supplier :

Alomone Labs

Target :

Magnesium transporter NIPA4, NIPA-like protein 4, NIPA-like domain containing 4, Ichthyin, ICHYN, ICHN, Non-imprinted in Prader-Willi/Angelman syndrome region protein 4

Short Description :

A Rabbit Polyclonal Antibody to Magnesium Transporter NIPA4

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human magnesium transporter NIPA4. Anti-NIPAL4 (extracellular) Antibody (#ANT-144) can be used in western blot and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the transporter in living cells. It has been designed to recognize NIPAL4 from mouse, rat and human samples.

Negative Control :

BLP-NT144

Positive Control :

Synonyms :

Magnesium transporter NIPA4, NIPA-like protein 4, NIPA-like domain containing 4, Ichthyin, ICHYN, ICHN, Non-imprinted in Prader-Willi/Angelman syndrome region protein 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

NIPAL4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage