antibody

Anti-NIPA2 (extracellular) Antibody

ANT-142

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunocytochemistry

ANT-142-CF_0.2 ml

Anti-NIPA2 (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q9JJC8

IC IF LCI WB

Human Rat Mouse

Rabbit

BLP-NT142

Rat, human - identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

2nd extracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)ETLNEMSHKLGD, corresponding to amino acid residues 137-148 of mouse magnesium transporter NIPA2

The NIPA family is currently comprised of 4 members: NIPA1-4. These members have a molecular similarity of 40%. They are integral membrane proteins which function as Mg2+ transporters.While NIPA2 is highly selective for Mg2+ transportation, other members of the family transport other cations as well. NIPA2 has 9 transmembrane protein domains. It is also found in the early endosome and transfers extracellular Mg2+ into the cytoplasm. NIPA2 is highly conserved among vertebrates; human and mouse NIPA2 have a 96% amino acid identity. NIPA2 is expressed in the heart, CNS and pancreas. Its expression is upregulated in MDCT cells cultured in low-magnesium levels compared to normal magnesium.Several imprinted genes have been identified as candidates for prader-willi syndrome in the 15q11-q13 region however the involvement of NIPA2, which resides in this region, remains unclear. Currently there is no evidence that the gene is directly involved in the many complexities of the syndrome- Mouse models of prader-willi-like pathologies include severe failure to thrive and early postnatal lethality have not implicated NIPA21.NIPA2 has also been recently implicated in the pathogenesis of childhood absence epilepsy (CAE). NIPA2 mutations have been found in some CAE patients. These mutations comprise of 2 missense mutations and a small insertion mutation. All mutations are heterozygous and are inherited from the paternal side. These mutations affect the highly conserved amino-acid structure of the transporter2.

IC IF LCI WB

Alomone Labs

Magnesium transporter NIPA2, Non-imprinted in Prader-Willi/Angelman syndrome region protein 2 homolog

A Rabbit Polyclonal Antibody to Magnesium Transporter NIPA2

Alomone Labs is pleased to offer an antibody directed against an epitope of mouse magnesium transporter NIPA2. Anti-NIPA2 (extracellular) Antibody (#ANT-142) can be used in western blot and immunocytochemistry in living cells. It has been designed to recognize NIPA2 from rat, human and mouse samples.

BLP-NT142

NA

Magnesium transporter NIPA2, Non-imprinted in Prader-Willi/Angelman syndrome region protein 2 homolog

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

NIPA2

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

Contact Alomone for information

1:200