Anti-SLC47A1 Antibody | Alomone Labs ANT-131 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC47A1 Antibody

catalog :

ANT-131

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Balla A, Jeong Y, Kim H, Lee Y, Chung S, Chae Y, et al. Effects of 1α,25-Dihydroxyvitamin D3 on the Pharmacokinetics of Procainamide and Its Metabolite N-Acetylprocainamide, Organic Cation Transporter Substrates, in Rats with PBPK Modeling Approach. Pharmaceutics. 2021;13: pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat testis (lanes 3 and 6): - 1-3. Anti-SLC47A1 Antibody (#ANT-131), (1:200).4-6. Anti-SLC47A1 Antibody, preincubated with SLC47A1 Blocking Peptide (#BLP-NT131).

image 2 :

Expression of SLC47A1 (MATE1) in rat parietal cortex. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-SLC47A1 Antibody (#ANT-131), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. SLC47A1 (MATE1) immunoreactivity (green) appears in pyramidal neurons in the parietal cortex (arrows). B. Pre-incubation of the antibody with SLC47A1 Blocking Peptide (#BLP-NT131), suppressed staining. Cell nuclei are stained with DAPI (blue).

image 3 :

Alomone Labs ProTx-II-Biotin inhibits NaV1.7 channels expressed inXenopusoocytes. - A. Time course ofProTx-II-Biotin(#STP-100-B) inhibition of NaV1.7 channels current. Membrane potential was held at -100 mV current was elicited by a 100 ms voltage step to 0 mV every 10 sec and was significantly inhibited by 200 nM ProTx-II-Biotin (bar)applied for 4.5 min. B. Superimposed traces of NaV1.7 current upon application of control and of 200 nM ProTx-II-Biotin (as indicated) taken from the recording shown in A.

product information

CAT :

ANT-131

SKU :

ANT-131-CF_0.2 ml

Product Name :

Anti-SLC47A1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8K0H1

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT131

Homology :

Rat - identical; human - no apparent homology

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

5th intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)ESHGEIMMTDLEKK, corresponding to amino acid residues 496-509 of mouse SLC47A1

Product Page - Scientific background :

MATE1 (SLC47A1) and MATE2 (SLC47A2) are multidrug and toxin extrusion transporter 1 and 2 function as cationic pumps in the kidneys, liver, and intestine1. Both MATEs excrete molecules with a positive net-charge from renal tubular epithelial cells in the kidney (where they are co-expressed with organic anion- and cation-transporters; OATs and OCTs), and into the urine. In the liver, MATE1, but not MATE2, regulates the transportation of tetraethylammonium (TEA) and 1-methyl-4-phenylpyridinium (MPP) - again, by cation-powered pumps2.Fairly recent X-ray crystallography has revealed that mammalian MATE1 has 13 transmembrane helices and a long cytoplasmic tail. Cysteine and histidine residues are responsible for the protein's functionality1.Overexpression of MATE1 is helpful in the delivery of metal-based anti-cancer drugs, such as cisplatin and oxaliplatin, as it effectively accumulates platinum intracellularly3. Furthermore, a rare increase in copy number of MATE1 in maize protects the plant from aluminum (Al) toxicity4. On the other hand, inhibition of MATE1 may lead to potentially dangerous drug-drug interactions, due to damaged drug extrusion regulation5.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

MATE1, Multidrug and toxin extrusion protein 1, Multi antimicrobial extrusion protein

Short Description :

A Rabbit Polyclonal Antibody to Multidrug and Toxin Extrusion Protein 1 (MATE1, SLC47A1)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse Multidrug and toxin extrusion protein 1 (MATE1, SLC47A1). Anti-SLC47A1 Antibody (#ANT-131) can be used in western blot analysis. It has been designed to recognize SLC47A1 from rat and mouse samples. The antibody will not recognize SLC47A1 from human samples.

Negative Control :

BLP-NT131

Positive Control :

Synonyms :

MATE1, Multidrug and toxin extrusion protein 1, Multi antimicrobial extrusion protein

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC47A1

AB Specifictiy :

Will not recognize human SLC47A1.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage