Anti-UCP2 Antibody | Alomone Labs ANT-128 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-UCP2 Antibody

catalog :

ANT-128

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse heart (lanes 1 and 5), rat skeletal muscle (lanes 2 and 6), rat adipose tissue (lanes 3 and 7) and rat pancreas (lanes 4 and 8): - 1-4. Anti-UCP2 Antibody (#ANT-128), (1:200).5-8. Anti-UCP2 Antibody, preincubated with UCP2 Blocking Peptide (#BLP-NT128).

product information

CAT :

ANT-128

SKU :

ANT-128-CF_0.2 ml

Product Name :

Anti-UCP2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P56500

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT128

Homology :

Mouse - identical; human - 11/12 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GRRYQSTVEAYK, corresponding to amino acid residues 153-164 of rat mitochondrial uncoupling protein 2 (UCP2)

Product Page - Scientific background :

Mitochondrial carriers (MCs) transport a variety of metabolites across the inner mitochondrial membrane. All MCs belong to the same family since their polypeptide chains consist of three tandemly related sequences of about 100 amino acids. MCs are encoded in humans by SLC25 genes.UCP2 (Uncoupling protein 2) is a member of the MC family encoded by the SLC25A8 gene. It is expressed on the inner mitochondrial membrane of cells in the lungs, kidneys, spleen, heart and mainly in the hypothalamus, pituitary and brain stem. The expression of UCP2 is upregulated by fatty acids, prostaglandins, TNFα and other cytokines. UCP2 is involved in regulation of body temperature and the control of oxygen radical production.Each monomer of UCP2 has six helices crossing the cell membrane connected by hydrophilic loops with both the N- and C- termini on the cytosolic side of the inner mitochondrial membrane. UCP2 catalyzes the unidirectional transport of H+. Charge balance can be achieved by cotransport (symport) of solutes, counter-transport of solutes and uniport of electroneutral metabolites. The transport of H+ is stimulated by fatty acids and inhibited by purine nucleotides. In addition, activation of H+ transport by superoxidase has been observed.Recent studies suggest that UCP2 and other uncoupling proteins are involved in pathologies such as obesity, type-2 diabetes and atherosclerosis. Mice lacking UCP2 have been shown to over-produce reactive oxygen but not to gain weight or be insensitive to cold stimuli1.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Mitochondrial uncoupling protein 2, UCPH, Solute carrier family 25 member 8, SLC25A8, BMIQ4

Short Description :

A Rabbit Polyclonal Antibody to Mitochondrial Uncoupling Protein 2

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat mitochondrial uncoupling protein 2 (UCP2). Anti-UCP2 Antibody (#ANT-128) can be used in western blot and immunocytochemistry applications. It has been designed to recognize UCP2 from human, rat and mouse samples.

Negative Control :

BLP-NT128

Positive Control :

Synonyms :

Mitochondrial uncoupling protein 2, UCPH, Solute carrier family 25 member 8, SLC25A8, BMIQ4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

UCP2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage