Anti-SLC25A18 (GC2) Antibody | Alomone Labs ANT-118 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC25A18 (GC2) Antibody

catalog :

ANT-118

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysates (lanes 1 and 4), rat brain lysates (lanes 2 and 5) and rat heart lysates (lanes 3 and 6):

image 2 :

Western blot analysis of human glioblastoma U-87 MG cell line lysate (lanes 1 and 3) and human brain neuroblastoma SH-SY5Y cell line lysate (lanes 2 and 4):

image 3 :

Expression of SLC25A18 in rat striatum:

product information

CAT :

ANT-118

SKU :

ANT-118-CF_0.2 ml

Product Name :

Anti-SLC25A18 (GC2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9DB41

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT118

Homology :

Rat – identical Human – 13 out of 16 amino acid identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, 1st loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RQLLMQDGTQRNLKME, corresponding to amino acid residues 95-110 of mouse SLC25A18

Product Page - Scientific background :

The solute carrier family 25 member 18 (SLC25A18) is one of the mitochondrial glutamate carriers within the SLC25 family, also known as the mitochondrial carrier family (MCF). Alongside SLC25A22, which shares similar structural and functional properties, SLC25A18 facilitates the transport of glutamate across the mitochondrial inner membrane by functioning as a symporter, coupling glutamate transport with proton movement to maintain mitochondrial pH and ionic balance1,3. SLC25A18 is predominantly expressed in the brain, liver, and testis, with comparatively lower levels in tissues like the breast, lung, and colon2,3. Its tissue-specific expression aligns with its functional role in nitrogen metabolism and the aspartate-glutamate shuttle, which connects mitochondrial and cytosolic processes critical for energy production and amino acid metabolism1,3. Biological processes involving SLC25A18 include energy metabolism and regulation of the Warburg effect. For instance, in colorectal cancer (CRC), SLC25A18 inhibits the Warburg effect and reduces cell proliferation via the Wnt/β-catenin pathway2. In Alzheimer’s disease (AD), elevated SLC25A18 expression contributes to mitochondrial dysfunction and neuronal apoptosis, emphasizing its role in neurodegeneration4,5. These processes highlight the significance of maintaining proper SLC25A18 activity for cellular homeostasis. Dysregulation of SLC25A18 is associated with conditions such as colorectal cancer and neurodegenerative diseases. Immunodetection of SLC25A18 can benefit research into cancer biology, mitochondrial dysfunction, and neurodegeneration, offering insights into both metabolic and signaling pathways relevant to these diseases.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Mitochondrial glutamate carrier 2, Glutamate/H(+) symporter 2, Solute carrier family 25 member 18, GC-2

Short Description :

A Rabbit polyclonal antibody to SLC25A18 (GC2)

Long Description :

Anti-SLC25A18 (GC2) Antibody (#ANT-118) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC25A18 from mouse, rat and human samples.

Negative Control :

BLP-NT118

Positive Control :

Synonyms :

Mitochondrial glutamate carrier 2, Glutamate/H(+) symporter 2 Solute carrier family 25 member 18, GC-2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC25A18

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

more info or order :

Alomone Labs product webpage