Anti-xCT/SLC7A11 (extracellular) Antibody | Alomone Labs ANT-111 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-xCT/SLC7A11 (extracellular) Antibody

catalog :

ANT-111

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 6

Reference
Chang I, Ohn T, Moon D, Maeng Y, Jang B, Yoon S. SNU-333 Cells as an Appropriate Cell Line for the Orthotopic Renal Cell Carcinoma Model. Technol Cancer Res Treat. 2021;20:15330338211038487 pubmed publisher
Eleftheriadis T, Pissas G, Filippidis G, Liakopoulos V, Stefanidis I. Reoxygenation induces reactive oxygen species production and ferroptosis in renal tubular epithelial cells by activating aryl hydrocarbon receptor. Mol Med Rep. 2021;23: pubmed publisher
Eleftheriadis T, Pissas G, Nikolaou E, Filippidis G, Liakopoulos V, Stefanidis I. Mistimed H2S upregulation, Nrf2 activation and antioxidant proteins levels in renal tubular epithelial cells subjected to anoxia and reoxygenation. Biomed Rep. 2020;13:3 pubmed publisher
Eleftheriadis T, Pissas G, Nikolaou E, Liakopoulos V, Stefanidis I. The H2S-Nrf2-Antioxidant Proteins Axis Protects Renal Tubular Epithelial Cells of the Native Hibernator Syrian Hamster from Reoxygenation-Induced Cell Death. Biology (Basel). 2019;8: pubmed publisher
Eleftheriadis T, Pissas G, Liakopoulos V, Stefanidis I. Factors that May Protect the Native Hibernator Syrian Hamster Renal Tubular Epithelial Cells from Ferroptosis Due to Warm Anoxia-Reoxygenation. Biology (Basel). 2019;8: pubmed publisher
Zhang H, Shen H, Jordan C, Liu Q, Gardner E, Bonci A, et al. CB2 receptor antibody signal specificity: correlations with the use of partial CB2-knockout mice and anti-rat CB2 receptor antibodies. Acta Pharmacol Sin. 2019;40:398-409 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat stomach (lanes 3 and 6) lysates: - 1-3. Anti-xCT/SLC7A11 (extracellular) Antibody (#ANT-111), (1:800).4-6. Anti-xCT/SLC7A11 (extracellular) Antibody, preincubated with xCT/SLC7A11 (extracellular) Blocking Peptide (#BLP-NT111).

image 2 :

Expression of xCT/SLC7A11 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-xCT/SLC7A11 (extracellular) Antibody (#ANT-111), (1:300), followed by goat anti-rabbit-AlexaFluor-488. A. SLC7A11 staining in hippocampal CA1 region is detected in the pyramidal layer (P, vertical arrows) and in glial profiles in stratum radiatum (SR, horizontal arrows). B. Pre-incubation of the antibody with xCT/SLC7A11 (extracellular) Blocking Peptide (#BLP-NT111), suppresses staining. Cell nuclei are stained with DAPI (blue). P = Pyramidal layer; SR = Stratum radiatum; SO = Stratum oriens.

product information

CAT :

ANT-111

SKU :

ANT-111-CF_0.2 ml

Product Name :

Anti-xCT/SLC7A11 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WTR6

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT111

Homology :

Rat - identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)THHFKDAFSGRDTS, corresponding to amino acid residues 218-231 of mouse cystine/glutamate transporter

Product Page - Scientific background :

The SLC7 family is divided into two subgroups, the cationic amino acid transporters (the CAT family, SLC7A1-4) and the glycoprotein-associated amino acid transporters (the gpaAT family, SLC7A5-11) also called light chains or catalytic chains of the hetero(di)meric amino acid transporters (HAT). The gene SLC7A11 encodes the xCT protein (also known as Cystine/glutamate transporter).xCT, as well as other HATs, is comprised of a light chain with 12 non-glycosylated transmembrane helices and is associated with the glycosylated heavy chain 4F2hc (CD98, SLC3A2) or rBAT (SLC3A1) through a conserved disulphide bridge. The COOH-terminus of the xCT is localized intracellularly. Association of the light and heavy chains is required for surface expression of the transporter which forms the heterodimeric amino acid transport system xc−. xCT is mainly expressed macrophages, brain, retinal pigment cells, liver and kidney. xCT expression is elevated in cells requiring high glutathione synthesis, such as activated macrophages, neuronal and glial cells and other cells after glutathione depletion1.xCT operates in a Na+-independent and electroneutral mode, exchanging extracellular anionic cystine (with pH dependence) for glutamate with a stoichiometry of 1:1. The driving force for this exchange is generated by the cystine concentration gradient (intracellular reduction) and the high intracellular concentration of glutamate.xCT has been suggested as a mediator of treatment resistance in cancer patients. In tumor cells, xCT plays a crucial role in regulating intracellular levels of glutathione which has been broadly implicated in resistance to chemotherapy2.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1

Short Description :

A Rabbit Polyclonal Antibody to Cystine/Glutamate Transporter (xCT)

Long Description :

Anti-xCT/SLC7A11 (extracellular) Antibody (#ANT-111) is a highly selective antibody directed against an epitope of the mouse cystine/glutamate transporter. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize xCT from human, mouse, and rat samples.

Negative Control :

BLP-NT111

Positive Control :

Synonyms :

Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC7A11

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:300

Application Dilutions: Western blot wb :

1:200-1:800

more info or order :

Alomone Labs product webpage