Anti-xCT/SLC7A11 (extracellular)-FITC Antibody | Alomone Labs ANT-111-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-xCT/SLC7A11 (extracellular)-FITC Antibody

catalog :

ANT-111-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of cystine/glutamate transporter in live intact mouse J774 macrophage cells: Cell surface detection of cystine/glutamate transporter in live intact mouse J774 macrophage cells: Black line: Cells. Red line: Cells + rabbit IgG isotype control-FITC. Green line: Cells + Anti-xCT/SLC7A11 (extracellular)-FITC Antibody (#ANT-111-F), (5 µg).

product information

CAT :

ANT-111-F

SKU :

ANT-111-F_15 mcl

Product Name :

Anti-xCT/SLC7A11 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WTR6

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT111

Homology :

Rat - identical; human - 11/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

3rd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)THHFKDAFSGRDTS, corresponding to amino acid residues 218-231 of mouse cystine/glutamate transporter

Product Page - Scientific background :

The SLC7 family is divided into two subgroups, the cationic amino acid transporters (the CAT family, SLC7A1-4) and the glycoprotein-associated amino acid transporters (the gpaAT family, SLC7A5-11) also called light chains or catalytic chains of the hetero(di)meric amino acid transporters (HAT). The gene SLC7A11 encodes the xCT protein (also known as Cystine/glutamate transporter).xCT, as well as other HATs, is comprised of a light chain with 12 non-glycosylated transmembrane helices and is associated with the glycosylated heavy chain 4F2hc (CD98, SLC3A2) or rBAT (SLC3A1) through a conserved disulphide bridge. The COOH-terminus of the xCT is localized intracellularly. Association of the light and heavy chains is required for surface expression of the transporter which forms the heterodimeric amino acid transport system xc−. xCT is mainly expressed macrophages, brain, retinal pigment cells, liver and kidney. xCT expression is elevated in cells requiring high glutathione synthesis, such as activated macrophages, neuronal and glial cells and other cells after glutathione depletion1.xCT operates in a Na+-independent and electroneutral mode, exchanging extracellular anionic cystine (with pH dependence) for glutamate with a stoichiometry of 1:1. The driving force for this exchange is generated by the cystine concentration gradient (intracellular reduction) and the high intracellular concentration of glutamate.xCT has been suggested as a mediator of treatment resistance in cancer patients. In tumor cells, xCT plays a crucial role in regulating intracellular levels of glutathione which has been broadly implicated in resistance to chemotherapy2.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1

Short Description :

A Rabbit Polyclonal Antibody to Cystine/Glutamate Transporter (xCT) Conjugated to the Fluorescent dye FITC

Long Description :

Anti-xCT/SLC7A11 (extracellular) Antibody (#ANT-111) is a highly selective antibody directed against an epitope of the mouse cystine/glutamate transporter. The antibody can be used in western blot analysis. It has been designed to recognize xCT from human, mouse, and rat samples. Anti-xCT/SLC7A11 (extracellular)-FITC Antibody (#ANT-111-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Cystine/glutamate transporter, Amino acid transport system xc-, Solute carrier family 7 member 11, Calcium channel blocker resistance protein CCBR1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC7A11

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANT-111), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage