Anti-LAT2 (SLC7A8) (extracellular) Antibody | Alomone Labs ANT-108 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LAT2 (SLC7A8) (extracellular) Antibody

catalog :

ANT-108

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysates (lanes 1 and 3) and rat brain synaptosomal fraction (lanes 2 and 4): - 1-2. Anti-LAT2 (SLC7A8)(extracellular) Antibody (#ANT-108), (1:200). 3-4. Anti-LAT2 (SLC7A8)(extracellular) Antibody, preincubated with LAT2 (SLC7A8)(extracellular) Blocking Peptide (BLP-NT108).

image 2 :

Expression of SLC7A8 in mouse hippocampus. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-LAT2 (SLC7A8)(extracellular) Antibody (#ANT-108), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Staining in the mouse hippocampal dentate gyrus region, showed immunoreactivity of SLC7A8 (green) in the hilus (H). B. Pre-incubation of the antibody with LAT2 (SLC7A8)(extracellular) Blocking Peptide (BLP-NT108), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANT-108

SKU :

ANT-108-CF_0.2 ml

Product Name :

Anti-LAT2 (SLC7A8) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WVR6

Applications :

IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT108

Homology :

Mouse - identical; Human - 15 out of 16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, 3rd loop.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CKGEFFWLEPKNAFEN, corresponding to amino acid residues 211 - 226 of rat SLC7A8

Product Page - Scientific background :

L-type amino acid transporter (LAT) family are transporters responsible for the uptake of neutral amino acids into cells. The LATs family contain four different members LAT1 (SLC7A5), LAT2 (SLC7A8), LAT3 (SLC43A1) and LAT4 (SLC43A2). LATs transporters are known to carried out their function in an Na+ and pH independent manner1. In recent years, LATs family shown to participate in the uptake of thyroid hormones (THs) and their derivatives2. LAT2 (SLC7A8) is a transmembrane protein first discovered back in 1999 using sequence similarity to LAT1. According to the predicted membrane topology, LAT2 consists of 12 transmembrane domains (TMDs) and N- and C- termini located in the cytosol. LAT2 associate with the 4F2hc (4F2 antigen heavy chain; CD98 heavy chain) glycoprotein to form a dimer that act as a neutral amino acid transporter3. LAT2 is expressed in various tissues, including the intestinal wall, blood-brain barrier, and kidney. Mutations in LAT2 protein cause age-related hearing loss in mice and humans5. In addition, deletion LAT2 in mice led to increased incidence of cataract4,5.

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Large Neutral Amino Acids Transporter Small Subunit 2, L-Type Amino Acid Transporter 2, Solute carrier family 7 member 8

Short Description :

A Rabbit Polyclonal antibody to LAT2 (SLC7A8) (extracellular)

Long Description :

Anti-LAT2 (SLC7A8) (extracellular) Antibody (#ANT-108) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize SLC7A8 from rat, mouse and human samples.

Negative Control :

BLP-NT108

Positive Control :

Synonyms :

Large Neutral Amino Acids Transporter Small Subunit 2, L-Type Amino Acid Transporter 2, Solute carrier family 7 member 8

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC7A8

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage