Anti-LAT2 (SLC7A8) (extracellular)-FITC Antibody | Alomone Labs ANT-108-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LAT2 (SLC7A8) (extracellular)-FITC Antibody

catalog :

ANT-108-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of LAT2 (SLC7A8) by direct flow cytometry in live intact mouse P815 mastocytoma cell line:

image 2 :

Cell surface detection of LAT2 (SLC7A8) by direct flow cytometry in live intact human Jurkat T-cell leukemia cell line:

product information

CAT :

ANT-108-F

SKU :

ANT-108-F_15 mcl

Product Name :

Anti-LAT2 (SLC7A8) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WVR6

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT108

Homology :

Mouse - identical; Human - 15 out of 16 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular, 3rd loop.

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CKGEFFWLEPKNAFEN, corresponding to amino acid residues 211 - 226 of rat SLC7A8

Product Page - Scientific background :

L-type amino acid transporter (LAT) family are transporters responsible for the uptake of neutral amino acids into cells. The LATs family contain four different members LAT1 (SLC7A5), LAT2 (SLC7A8), LAT3 (SLC43A1) and LAT4 (SLC43A2). LATs transporters are known to carried out their function in an Na+ and pH independent manner1. In recent years, LATs family shown to participate in the uptake of thyroid hormones (THs) and their derivatives2. LAT2 (SLC7A8) is a transmembrane protein first discovered back in 1999 using sequence similarity to LAT1. According to the predicted membrane topology, LAT2 consists of 12 transmembrane domains (TMDs) and N- and C- termini located in the cytosol. LAT2 associate with the 4F2hc (4F2 antigen heavy chain; CD98 heavy chain) glycoprotein to form a dimer that act as a neutral amino acid transporter3. LAT2 is expressed in various tissues, including the intestinal wall, blood-brain barrier, and kidney. Mutations in LAT2 protein cause age-related hearing loss in mice and humans5. In addition, deletion LAT2 in mice led to increased incidence of cataract4,5.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Large Neutral Amino Acids Transporter Small Subunit 2, L-Type Amino Acid Transporter 2, Solute carrier family 7 member 8

Short Description :

A Rabbit Polyclonal antibody to LAT2 (SLC7A8) (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-LAT2 (SLC7A8) (extracellular) Antibody (#ANT-108) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize SLC7A8 from rat, mouse and human samples. Anti-LAT2 (SLC7A8) (extracellular)-FITC Antibody (#ANT-108-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Large Neutral Amino Acids Transporter Small Subunit 2, L-Type Amino Acid Transporter 2, Solute carrier family 7 member 8

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC7A8

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANT-108), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage