Anti-LAT1 (SLC7A5) Antibody | Alomone Labs ANT-105 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-LAT1 (SLC7A5) Antibody

catalog :

ANT-105

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat placenta lysate (lanes 1 and 4), mouse testes lysate (lanes 2 and 5) and rat skeletal muscle membrane (lanes 3 and 6): - 1-3. Anti-LAT1 (SLC7A5) Antibody (#ANT-105), (1:200).4-6. Anti-LAT1 (SLC7A5) Antibody, preincubated with LAT1/SLC7A5 Blocking Peptide (#BLP-NT105).

image 2 :

Expression of LAT1/SLC7A5 in rat dorsal root ganglion. - Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections with Anti-LAT1 (SLC7A5) Antibody (#ANT-105), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. LAT1 immunoreactivity (green) appeared in neuron profiles (arrows). B. Pre-incubation of the antibody with LAT1 (SLC7A5) Blocking Peptide (#BLP-NT105), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANT-105

SKU :

ANT-105-CF_0.2 ml

Product Name :

Anti-LAT1 (SLC7A5) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63016

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT105

Homology :

Mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

3rd intracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KDIGQGDASNLHQK, corresponding to amino acid residues 222-235 of rat LAT1

Product Page - Scientific background :

L-type amino acid transporter 1 (LAT1) is responsible for mediating the transport of amino acids into highly proliferative cells. LAT1 is Na+-independent amino acid transporter that transports branched-chain and aromatic neutral amino acids including isoleucine, histidine, leucine, methionine, phenylalanine, tryptophan, tyrosine, methionine and valine. LAT1 forms heterodimeric complexes with the 4F2 cell-surface antigen heavy chain (4F2hc) via a disulfide bond 1,2. Structurally, LAT1 has 12 membrane spanning domains with intracellular N- and C-terminal tails3.LAT1 mRNA expression can be found in various tissues such as the brain, spleen, placenta, and testis. High protein levels are particularly detected in pancreatic islet. In cells, LAT1 is localized to the basolateral membrane of polarized epithelia1,2.Studies show that upregulation in LAT1 protein is linked and associated with cancer cell proliferation and is highly expressed in tumor cell lines including oral squamous cell carcinoma, esophageal carcinoma, gastric cancer, prostate cancer, non-small cell lung carcinoma, biliary tract cancer, pancreatic cancer, and breast cancer2.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

CD98, Large neutral amino acids transporter small subunit 1, L-type amino acid transporter 1, 4F2 light chain, 4F2LC, Integral membrane protein E16, MPE16, Solute carrier family 7 member 5, TA1, Tumor-associated protein 1

Short Description :

A Rabbit Polyclonal Antibody to LAT1

Long Description :

Anti-LAT1 (SLC7A5) Antibody (#ANT-105) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize SLC7A5 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Negative Control :

BLP-NT105

Positive Control :

Synonyms :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC7A5

AB Specifictiy :

Not recommended for use with human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage