Anti-SLC7A2 (extracellular)-FITC Antibody | Alomone Labs ANT-103-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC7A2 (extracellular)-FITC Antibody

catalog :

ANT-103-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of SLC7A2 in live intact human THP-1 monocytic leukemia cells: Cell surface detection of SLC7A2 in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Black line: Cells + rabbit IgG isotype control-FITC. Black line: Cells + Anti-SLC7A2 (extracellular)-FITC Antibody (#ANT-103-F), (5 µg).

product information

CAT :

ANT-103-F

SKU :

ANT-103-F_50 mcl

Product Name :

Anti-SLC7A2 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

B5D5N9

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT103

Homology :

Mouse - identical; human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KTYFKMNYTGLAE, corresponding to amino acid residues 151-163 of rat SLC7A2

Product Page - Scientific background :

The cationic amino acid transporter (CAT) family is comprised of four homologous membrane proteins: CAT-1, CAT-2, CAT-3 and CAT-4, encoded by the SLC7A1−4 genes, respectively. The major difference between these transporters is at the level of tissue specificity and regulation of their expression1,2.The CAT family is included in the Na+-independent transport system, (named system y+) that mediate L-arginine uptake in most tissues and cells3.CAT-2 (SLC7A2) is known to transport cationic amino acids such as L-arginine, L-ornithine, and L-lysine. It is considered to be the major arginine transporter in most cells and tissues. It can be expressed in two spliced variants, CAT-2A and CAT-2B. CAT-2A, is a low-affinity transporter, constitutively expressed in liver and muscle cells, whereas CAT-2B, is a high-affinity transporter, abundantly expressed in macrophages3.Macrophages isolated from mice deficient in CAT-2 show impaired ability to transport arginine intracellularly and to produce nitric oxide in response to endotoxin, thus CAT2 may also be important in regulating immune cell function in inflammation4.Studies have identified CAT2 as a regulator of fibrotic responses in the lung5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Cationic amino acid transporter 2, CAT-2, Low affinity cationic amino acid transporter 2, ATRC2, Solute carrier family 7 member 2

Short Description :

A Rabbit Polyclonal Antibody to Cationic Amino Acid Transporter 2 (SLC7A2) Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-SLC7A2 (extracellular) Antibody (#ANT-103) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize Cationic amino acid transporter 2 from mouse, rat, and human samples. Anti-SLC7A2 (extracellular)-FITC Antibody (#ANT-103-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Cationic amino acid transporter 2, CAT-2, Low affinity cationic amino acid transporter 2, ATRC2, Solute carrier family 7 member 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC7A2

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANT-103), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage