Anti-SLC3A1 (extracellular) Antibody | Alomone Labs ANT-100 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC3A1 (extracellular) Antibody

catalog :

ANT-100

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lane 1 and 3) and mouse (lanes 2 and 4) kidney lysates: - 1,2. Anti-SLC3A1 (extracellular) Antibody (#ANT-100), (1:400).3,4. Anti-SLC3A1 (extracellular) Antibody, preincubated with SLC3A1 (extracellular) Blocking Peptide (#BLP-NT100).

product information

CAT :

ANT-100

SKU :

ANT-100-CF_0.2 ml

Product Name :

Anti-SLC3A1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q64319

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT100

Homology :

Mouse - identical; human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KEQPDLNFRNPAVQE, corresponding to amino acid residues 277-291 of rat SLC3A1

Product Page - Scientific background :

The heteromeric amino acid transporter (HAT) family is one of the major amino acid transporter families responsible for cellular uptake and epithelial transport. The uptake of cysteine mainly depends on HATs. HATs are localized to the plasma membrane and form heterodimers composed of a 12-membrane spanning light chain (SLC7) and a single membrane spanning heavy chain, SLC3. SLC7 catalyzes transport functions while SLC3 is essential for plasma membrane localization and stabilization of the light chains. The SLC3A1 is mainly expressed in the apical plasma membrane in the epithelial cells of intestinal mucosa and the renal proximal tubules1,2. Two heavy chains, SLC3A1 and SLC3A2 are covalently bound to the light chains through a disulfide bridge. SLC3A1 has been known to form a heterodimer only with SLC7A9, a family member of light chain1,2.Type 1 Cystinuria is associated with mutations in SLC3A1, an inherited disease characterized by the formation of cystine calculi in the kidneys, ureters, and bladder3.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Neutral and basic amino acid transport protein rBAT, Solute carrier family 3 (cystine, dibasic and neutral amino acid transporters) member 1, B(0,+)-type amino acid transport protein, NBAT, Amino acid transporter 1, ATR1

Short Description :

A Rabbit Polyclonal Antibody to SLC3A1

Long Description :

Anti-SLC3A1 (extracellular) Antibody (#ANT-100) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize SLC3A1 from rat, mouse, and human samples.

Negative Control :

BLP-NT100

Positive Control :

Synonyms :

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC3A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage