antibody

Anti-SLC17A9 Antibody

ANT-085

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

ANT-085-CF_0.2 ml

Anti-SLC17A9 Antibody

Antibodies

Antibodies

Polyclonal

Q8VCL5

IF IHC WB

Human Rat Mouse

Rabbit

BLP-NT085

Human - 13/14 amino acid residues identical; rat - 12/14 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Intracellular, 2nd loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)SQKVQESERAFTYS, corresponding to amino acid residues 155-168 of mouse VNUT

The vesicular nucleotide transporter (VNUT), also known as SLC17A9, is a member of the solute carrier superfamily, which contains ~300 proteins. Members of the SLC17 family are expressed in intracellular membranes and insulated vesicles such as the lysosomes1.SLC17A9 has 430 amino acids and predicted to have 12 transmembrane domains with both the carboxyl- and N-termini facing the same direction5. Although the human SLC17A9 ortholog displays sequence-specific modification of its N-terminus, SLC17A9 is highly conserved across species, which is believed to dictate its substrate preference5.SLC17A9 is widely expressed in many tissues, especially in ATP containing vesicles4. In the brain, SLC17A9 is highly expressed in different regions such as the cortex, cerebellum, hippocampus, and olfactory bulbs, but also in astrocytes and microglia cells. SLC17A9 expression is also evident outside the central nervous system, such as in the gastrointestinal tract and the lungs4.Unlike other SLC17 members, SLC17A9 is highly specific for phosphorylated nucleotides with particularly high affinity for ATP3. Silencing of SLC17A9 in PC12 cells revealed its fundamental role in ATP homeostasis as it dramatically reduced ATP concentrations in storage and secretory vesicles. In addition, SLC17A9 also plays a role in the immune system where it activates neutrophils by influencing ATP storage in secretory vesicles2.Under specific circumstances, SLC17A9 is involved in different pathological conditions. For example, in the spinal cord, SLC17A9 expression is associated with a general incline to feel pain3. Moreover, SLC17A9 is also involved in the development of glaucoma in mice, possibly by regulating ATP secretion3.

IF IHC WB

Alomone Labs

Vesicular nucleotide transporter, VNUT

A Rabbit Polyclonal Antibody to Vesicular Nucleotide Transporter

Anti-SLC17A9 Antibody (#ANT-085) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize VNUT from human, rat, and mouse samples.

BLP-NT085

NA

Vesicular nucleotide transporter, VNUT

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SLC17A9

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:2000

1:200-1:400