Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody | Alomone Labs ANT-083-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody

catalog :

ANT-083-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of ASCT2 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells: Cell surface detection of ASCT2 by direct flow cytometry in live intact human Jurkat T-cell leukemia cells: Black line: Cells. Red line: Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F). Green line: Cells + Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody (#ANT-083-F), (2.5µg).

product information

CAT :

ANT-083-F

SKU :

ANT-083-F_15 mcl

Product Name :

Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q15758

Applications :

FC LCI

Reactivity :

Human

Host :

Rabbit

Blocking Peptide :

BLP-NT083

Homology :

Human only Not recommended for use with rat and mouse samples

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

2nd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RSYSTTYEERNITGTR, corresponding to amino acid residues 202 - 217 of human ASCT2

Product Page - Scientific background :

ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways. It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2

Short Description :

A Rabbit Polyclonal Antibody to SLC1A5 (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-Human ASCT2/SLC1A5 (extracellular) Antibody (#ANT-083) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize SLC1A5 from human samples only. The antibody will not recognize rat or mouse ASCT2. Anti-Human ASCT2/SLC1A5 (extracellular)-FITC Antibody (#ANT-083-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize SLC1A5 from human samples only. The antibody will not recognize rat or mouse ASCT2.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Alanine/Serine/Cysteine Transporter 2, Neutral amino acid transporter B(0), ATB(0), Sodium-dependent Neutral Amino Acid Transporter Type 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC1A5

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #ANT-083), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage