Anti-ASCT2 (SLC1A5) Antibody | Alomone Labs ANT-082 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-ASCT2 (SLC1A5) Antibody

catalog :

ANT-082

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain membranes (lanes 2 and 4): - 1,2. Anti-ASCT2 (SLC1A5) Antibody (#ANT-082), (1:200).3,4. Anti-ASCT2 (SLC1A5) Antibody, preincubated with ASCT2/SLC1A5 Blocking Peptide (#BLP-NT082).

image 2 :

Expression of ASCT2 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-ASCT2 (SLC1A5) Antibody (#ANT-082), (1:200), followed by goat-anti-rabbit-AlexaFluor-488. ASCT2 staining appears in a layer (arrows) adjacent to the dentate granule layer (GL). Cell nuclei are stained with DAPI (blue).

product information

CAT :

ANT-082

SKU :

ANT-082-CF_0.2 ml

Product Name :

Anti-ASCT2 (SLC1A5) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51912

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT082

Homology :

Rat - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)KMPSSEPELIQVKNE, corresponding to amino acid residues 502-516 of mouse SLC1A5

Product Page - Scientific background :

ASCT2 is an alanine-serine-cysteine amino acid transporter and solute linked carrier family 1 member A5 (SLC1A5). It functions as an essential high-affinity glutamine transporter. It also functions as a Na+-dependent transporter for alanine, serine and cysteine. The transport process is electroneutral which involves the Na+-coupled influx of glutamine to the Na+-coupled efflux of another substrate such as alanine, serine or cysteine. ASCT2 is expressed various peripheral tissues such as the lung and kidney1,2. ASCT2 has an important contribution to the homeostasis of amino acid metabolism within tumor cells3.ASCT2 seems to be up regulated in several cancerous tissues such as stomach, liver, kidney, breast, prostate, skin, colorectal, pancreatic, tongue and lung cancers. Studies have shown that ASCT2 is necessary for activating critical survival and cellular growth signaling cascades in cancer, which includes mTOR and ERK pathways. It has been shown that knockdown of ASCT2 in prostate cancer cell line xenograft models significantly inhibit tumor growth and metastasis in vivo. Thus, ASCT2 is a potential cancer therapeutic target1,2.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Alanine/serine/cysteine transporter 2, Neutral amino acid transporter B(0), ATB(0), ATBO, Sodium-dependent neutral amino acid transporter type 2, AAAT

Short Description :

A Rabbit Polyclonal Antibody to ASCT2 Transporter

Long Description :

Anti-ASCT2 (SLC1A5) Antibody (#ANT-082) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry analysis. It has been designed to recognize SLC1A5 from mouse and rat samples. It will not recognize human ASCT2.

Negative Control :

BLP-NT082

Positive Control :

Synonyms :

Alanine/serine/cysteine transporter 2, Neutral amino acid transporter B(0), ATB(0), ATBO, Sodium-dependent neutral amino acid transporter type 2, AAAT

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC1A5

AB Specifictiy :

Will not recognize ASCT2 from human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage