antibody

Anti-ASCT1 (SLC1A4) Antibody

ANT-081

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

ANT-081-CF_0.2 ml

Anti-ASCT1 (SLC1A4) Antibody

Antibodies

Antibodies

Polyclonal

O35874

IF IHC WB

Human Rat Mouse

Rabbit

BLP-NT081

Rat - identical; human - 12/13 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Intracellular, C-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

(C)SKSEEETSPLVTH, corresponding to amino acid residues 500-512 of mouse SLC1A4

Glutamate transporters play important roles in the termination of excitatory neurotransmission and in providing cells throughout the body with glutamate for metabolic purposes.SLC1A4 belongs to the SLC1 protein family which contains a range of human and prokaryotic glutamate and aspartate transporters such as the human glutamate transporters (otherwise known as excitatory amino acid transporters or EAATs).SLC1A4 is detected in the brain, skeletal muscle, and pancreas. It is largely selective for alanine, serine, and cysteine but can also transport threonine, asparagine, and to a lesser extent, proline. In the brain, SLC1A4 is preferentially expressed in glial cells and has been suggested to be responsible for the release of L-serine from intracellular stores, in exchange for other extracellular substrates. SLC1A4 exhibits pH-dependent substrate selectivity. Acidic amino acids become substrates at pH 5.5, which may be physiologically important in the acidic environment of the digestive tract where SLC1A4 is involved in nutrient uptake.Despite the high degree of sequence identity, ASCTs function quite differently from the EAATs. The EAATs transport the acidic amino acids glutamate and aspartate against a concentration gradient generating a net movement of substrate and charge across the membrane. In contrast, SLC1A4 exchanges small, neutral amino acids in an electroneutral fashion without generating net movement of substrate1.Recent studies have shown that mutations in the SCL1A4 gene, which are common in Ashkenazi Jews, are associated with global developmental delay, microcephaly and hypomyelination2.

IF IHC WB

Alomone Labs

Alanine/serine/cysteine/threonine transporter 1, Neutral amino acid transporter A, SATT

A Rabbit Polyclonal Antibody to ASCT1 Transporter

Anti-ASCT1 (SLC1A4) Antibody (#ANT-081) is a highly selective antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize SLC1A4 from human, mouse, and rat samples.

BLP-NT081

NA

Alanine/serine/cysteine/threonine transporter 1, Neutral amino acid transporter A, SATT

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

SLC1A4

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:300

1:200