Anti-Rat SLC4A5 (NBC4) Antibody | Alomone Labs ANT-076 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Rat SLC4A5 (NBC4) Antibody

catalog :

ANT-076

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Bhat V, Lee Wing V, Hu P, Raouf A. Isolation and characterization of a new basal-like luminal progenitor in human breast tissue. Stem Cell Res Ther. 2019;10:269 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and testis (lanes 2 and 4) membranes: - 1,2. Anti-Rat SLC4A5 (NBC4) Antibody (#ANT-076), (1:400).3,4. Anti-Rat SLC4A5 (NBC4) Antibody, preincubated with Rat SLC4A5/NBC4 Blocking Peptide (#BLP-NT076).

image 2 :

Western blot analysis of rat brain (lanes 1 and 3) and testis(lanes 2 and 4)membranes: - 12.Anti-Rat SLC4A5 (NBC4) Antibody(#ANT-076) (1:400).34. Anti-Rat SLC4A5 (NBC4) Antibody preincubated with the negative control antigen.

product information

CAT :

ANT-076

SKU :

ANT-076-CF_0.2 ml

Product Name :

Anti-Rat SLC4A5 (NBC4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6RI88

Applications :

Reactivity :

Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT076

Homology :

Rat only

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KLEPTSYRRRHPE, corresponding to amino acid residues 11-23 of rat NBCe2

Product Page - Scientific background :

The SLC4 transporter family, also known as the bicarbonate (HCO3-)-transporter family, includes the products of ten human genes (SLC4A1-5; A7-11). In mammals, the SLC4 membrane proteins are critical for several physiological processes, including the carriage of carbon dioxide (CO2) from the systemic capillaries to the pulmonary capillaries, the regulation of cell volume in multiple cell types, and the regulation of intracellular pH in nearly every cell of the body1.NBCe2 (NBC4) is an electrogenic sodium bicarbonate co-transporter encoded by SLC4A5. There are six NBCe2 isoform (SLC4A5A-F) reported2. NBCe2 is an integral membrane protein consisting of 14 transmembrane domains. NBCe2 has a long N-terminal hydrophilic domain and a much shorter C-terminal hydrophilic domain, both of which are intracellular3.NBCe2 is predominantly detected in the brain (highest in prefrontal cortex), epididymis, cardiac muscle, smooth muscle, kidney, and choroid plexus/apical1.Several studies identified SLC4A5 as a positional candidate for hypertension4,5. In mice, disruption of slc4a5 causes both arterial hypertension as well as metabolic acidosis (distal renal tubular acidosis)6. In addition, mice lacking NBCe2 have reduced choroid plexus secretion and reduced ventricle size. These combined issues change intracranial pressure, CSF electrolytes, and cause severe eye pathologies such as photoreceptor loss, ganglion cell loss, and retinal detachment7.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Electrogenic sodium bicarbonate cotransporter 4, NBCe2

Short Description :

A Rabbit Polyclonal Antibody to NBC4 Transporter

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NBCe2. Anti-Rat SLC4A5 (NBC4) Antibody (#ANT-076) can be used in western blot analysis. It has been designed to recognize NBCe2 from rat samples. The antibody is not recommended for mouse and human samples.

Negative Control :

BLP-NT076

Positive Control :

Synonyms :

Electrogenic sodium bicarbonate cotransporter 4, NBCe2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC4A5

AB Specifictiy :

Not recommended for human and mouse samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage