Anti-SLC4A4 (NBC1) (extracellular) Antibody | Alomone Labs ANT-075 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-SLC4A4 (NBC1) (extracellular) Antibody

catalog :

ANT-075

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-SLC4A4 (NBC1) (extracellular) Antibody (#ANT-075), (1:200).2. Anti-SLC4A4 (NBC1) (extracellular) Antibody, preincubated with SLC4A4/NBC1 (extracellular) Blocking Peptide (#BLP-NT075).

image 2 :

Expression of NBCe1 in rat lateral septum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-SLC4A4 (NBC1) (extracellular) Antibody (#ANT-075), (1:400). NBCe1 staining (red) is detected in blood vessel outlines (vertical arrow) in the lateral septum (LS) and along the wall of the lateral ventricle (LV) (horizontal arrow). DAPI is used as the counterstain (blue).

image 3 :

Peptide (C)KE(S)VKFGGKLVGNN corresponding to amino acid residues 672-685 of rat NBCe1 (AccessionQ9JI66). 2ndextracellular loop based on the 10 transmembrane topology.

product information

CAT :

ANT-075

SKU :

ANT-075-CF_0.2 ml

Product Name :

Anti-SLC4A4 (NBC1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JI66

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT075

Homology :

Rat - 13/14 amino acid residues identical; mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop, based on the 10 transmembrane topology.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KE(S)VKFGGKLVGNN, corresponding to amino acid residues 672-685 of rat NBCe1

Product Page - Scientific background :

SLC4 membrane transporter proteins play important roles in kidney acid-base regulation through their transport of bicarbonate (or carbonate), Na+, Cl-, and (possibly NH4+)1. These transporters differ in their substrate (Na+, Cl-) dependence, charge transport stoichiometry, cell-type and developmental expression, functional regulation, and protein-protein interactions. In mammals, SLC4 proteins are encoded by 10 different genes that share protein sequence homology and are grouped according to their functional properties.In humans, the electrogenic sodium bicarbonate cotransporter NBCe1 is encoded by the SLC4A4 gene2. NBCe1-A has a large N-terminal cytoplasmic region, a lipid embedded transmembrane region, and a C-terminal cytoplasmic tail3. The N-terminal cytoplasmic region is tightly folded and is predicted to form a domain structure, unlike the freely aqueous accessible C-terminal cytoplasmic tail. NBCe1-A is composed of either 10 or 14 transmembrane domains. Based on the 10 transmembrane topology, a large extracellular loop (EL) is present between TM3 and TM4 and contains two glycosylated sites. The oligomeric state of the cotransporter is dimeric and each monomeric subunit has independent transport activity4.Mutations in NBCe1 cause autosomal recessive proximal renal tubular acidosis (pRTA). Patients with NBCe1 mutations have severe pRTA, growth and mental retardation, basal ganglia calcification, cataracts, corneal opacities (band keratopathy), glaucoma, elevated serum amylase and lipase, and defects in the enamel consistent with amelogenesis imperfect3. Mice with loss of NBCe1 have a more severe phenotype with marked volume deletion and decreased survival5.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Electrogenic sodium bicarbonate cotransporter 1, NBCe1, NBC-like protein, Na+/HCO3- cotransporter

Short Description :

A Rabbit Polyclonal Antibody to NBC1 Transporter

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat NBCe1. Anti-SLC4A4 (NBC1) (extracellular) Antibody (#ANT-075) can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody was used to probe NBCe1 in living rat PC12 cells. It has been designed to recognize NBCe1 from rat and mouse samples and is not recommended for use in human samples.

Negative Control :

BLP-NT075

Positive Control :

Synonyms :

Electrogenic sodium bicarbonate cotransporter 1, NBCe1, NBC-like protein, Na+/HCO3- cotransporter

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC4A4

AB Specifictiy :

Not recommended for human samples. The antibody will recognize all known rat splice variants

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage