Anti-NKCC2 (SLC12A1) Antibody | Alomone Labs ANT-072 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-NKCC2 (SLC12A1) Antibody

catalog :

ANT-072

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Cherezova A, Tomilin V, Buncha V, Zaika O, Ortiz P, Mei F, et al. Urinary concentrating defect in mice lacking Epac1 or Epac2. FASEB J. 2019;33:2156-2170 pubmed publisher
Felix Ii R, Gourévitch B, Gómez Álvarez M, Leijon S, Saldaña E, Magnusson A. Octopus Cells in the Posteroventral Cochlear Nucleus Provide the Main Excitatory Input to the Superior Paraolivary Nucleus. Front Neural Circuits. 2017;11:37 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) kidney membranes: - 1,2. Anti-NKCC2 (SLC12A1) (extracellular) Antibody (#ANT-072), (1:400).3,4. Anti-NKCC2 (SLC12A1) (extracellular) Antibody, preincubated with NKCC2/SLC12A1 (extracellular) Blocking Peptide (#BLP-NT072).

product information

CAT :

ANT-072

SKU :

ANT-072-CF_0.2 ml

Product Name :

Anti-NKCC2 (SLC12A1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P55016

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-NT072

Homology :

Mouse - identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GYKKNWRKAPLSELE, corresponding to amino acid residues 776-790 of rat NKCC2

Product Page - Scientific background :

Na+-K+-2Cl− cotransporter (NKCC2) belongs to the family of integral plasma membrane proteins; solute carrier family 12 ,the electrically silent, cation-coupled chloride co-transporter family that mediates electroneutral symport of Na+, K+, and Cl− with a stochiometry of 1:1:2, respectively. NKCC2 structure contains a central hydrophobic domain made up of 12 putative transmembrane spanning regions and a long hydrophilic loop connects transmembrane segments 7 and 8 and contains two glycosylation sites. The hydrophobic domain is flanked by a short amino- and a long carboxyl-terminal domain that are located within the cell.NKCC2 encoded by the Slc12a1 gene shows at least six alternatively spliced variants and has the ability to form dimmers. NKCC2 activity and expression can be found in the medullary and cortical thick ascending limb of Henle's loop is the nephron and in macula densa cells.NKCC2 allows Na+ and Cl− entry from the tubule lumen into the thick ascending limb of Henle's loop, a process that allows reabsorbing 25-30% of the NaCl filtered by the glomeruli. In addition, NKCC2 can sense luminal Cl− concentration and initiates the signal for tubuloglomerular feedback in the specialized cells of the macula densa. Thus, NKCC2 activity is essential for salt conservation and for acute and chronic regulation of water balance.Mutations in the human NKCC2 gene causes Bartter syndrome type 1 characterized by severe natriuresis hypercalciuria and magnesuria, the inability to concentrate urine, leading to hyponatremia, hypochloremia, metabolic alkalosis, and low blood pressure. Opposite to Bartter's syndrome, enhanced activity of NKCC2 NaCl is connected with human and animal hypertension.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Na+-K+-2Cl- Cotransporter 2, Kidney-specific Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC1, Solute carrier family 12 member 1

Short Description :

A Rabbit Polyclonal Antibody to NKCC2 Transporter

Long Description :

Anti-NKCC2 (SLC12A1) Antibody (#ANT-072) is a highly specific antibody directed against an epitope of rat NKCC2 transporter. The antibody has been designed to recognize NKCC2 from rat, human and mouse samples.

Negative Control :

BLP-NT072

Positive Control :

Synonyms :

Na+-K+-2Cl- Cotransporter 2, Kidney-specific Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, BSC1, Solute carrier family 12 member 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

SLC12A1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage